1Dr.Shreedevi , 2Vd. B.R.Patel
DOI : http://dx.doi.org/10.31142/ijahm/v9i4.06
1PhD scholar, Department of Dravya Guna, Institute for Postgraduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat – 361 008, India.
2Asst Professor, Dept. of Dravyaguna, Institute for Postgraduate Teaching and Research in Ayurveda, Gujarat Ayurved University, Jamnagar, Gujarat – 361 008. India.
Abstract:-
Desmodium gangeticum DC. is the official drug considered for śālaparṇi. Previous survey study revealed use of Flemingia strobilifera(L). Aiton as śālaparṇi in Southern market. Due to the similarities in the leaves, it is difficult to differentiate the two market samples. Hence, the study was considered to know the genotype of both the plants with their similarities and differences. Tender leaves and their buds were utilized in this RAPD method. With minor modification, Doyle and Doyle (1990) method was used to extract the DNA. Verity ABI thermal cycler was used to carry out the RAPD-PCR. Under UV light in Gel document system, the resolved amplification products were visualized by illumination. As comparative analysis of the both species, banding pattern obtained in all the primers were matching at 500, 600 and 700bp range. In this range 9 bright bands from Desmodium gangeticum DC. and 7 bright bands from Flemingia strobilifera(L). Aiton were matching. These bands are specific for some morphological and some genetically inherited family characters.
Keywords: śālaparṇi, Flemingia strobilifera, Desmodium gangeticum
Reference
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2. Doyle JJ, Doyle JL. A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem Bull. 1987; 19:11–5.