Zúñiga-Martínez M L1, Terán-Figueroa Y2, Vértiz-Hernández A A3, Alcántara-Quintana L E4
DOI : http://dx.doi.org/10.31142/ijahm/v9i4.07
1Doctorado en Ciencias Biomédicas Básicas, Universidad Autónoma de San Luis Potosí
2Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí
3Coordinación Académica Región Altiplano, Universidad Autónoma de San Luis Potosí
4Cátedra CONACYT, adscrita a Facultad de Enfermería y Nutrición, Universidad Autónoma
de San Luis Potosí
*Corresponding author: Alcántara-Quintana L E
Cátedra CONACYT, adscrita a Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí
Abstract
Introduction: Some varieties of garlic - such as the Snow Mountain variety - have been attributed a greater amount of medicinal effects than the rest of the Allium genus, due mainly to the amount of sulfur compounds they contain. The amount of these compounds depends - among other circumstances - on the extraction conditions and techniques.
Objective: To assess the effects of Snow Mountain garlic extracts, obtained by different processes, on count and cell viability in Cervicouterine Cancer cell lines.
Material and methods: Three extraction methods were used to obtain sulfur compounds; three different concentrations of each extract were prepared and their effects were evaluated on two neoplastic and one non-neoplastic cell lines. The evaluation methods were Neubauer chamber cell count at 24, 48 and 72 hours and a MTT test at 72 hours.
Results: A significant dose-dependent increase in the number and viability of neoplastic cells was observed (p = 0.0001 and p = 0.0003 respectively), specially in soxhlet extraction treatments.
Conclusion: the ethanolic, chloroformic and aqueous Snow Mountain garlic extractions show an increase in the number and viability of Cervicouterine Cancer (CuCa) cells, contrary to other Allium varieties, so their probable anti-neoplastic activity requires further study.
Keywords: cancer, Allium sativum L., Snow Mountain garlic, cell count and viability.
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