1Jain Vandana, 2Patil Rashmi , 3Jaiswar Uttara
DOI : http://dx.doi.org/10.31142/ijahm/v9i5.03
Department of Quality Assurance, Orie ntal College of Pharmacy, Sanpada-Navi Mumbai, Maharashtra 400 705
ABSTRACT
A novel,simple, rapid, isocratic reverse phase high-performance liquid chromatographic method was developed for simultaneous estimation of andrographolide and berberine. The analysis was carried on Prontosil C18 (250 ×4.6 mm,5μ)column, with mobile phase consisting of acetonitrile and potassium dihydrogen phosphate buffer (pH 2.2 adjusted with ortho phosphoric acid) in the ratio of 30:70. The flow rate was set at 1.0ml/minand effluents were monitored at 235nm.The retention time of andrographolide and berberine werefound to be 10.08±0.2 and 12.47±0.2 minrespectively.Linear responses were obtained in concentration ranges of 5-50µg/ml, with R2 of 0.9989 and 0.9993 for andrographolide and berberine respectively. As per ICH guidelines, this method is accurate, simple, rapid and sensitive, hence can also be used in the evaluation of other formulations containing andrographolide and berberine.
Keywords: Reversephase high performance liquid chromatography, andrographolide, berberine,ICH guidelines.
REFERENCE
1. Kausik Biswas, Ishita Chattopadhyay, Ranajit K. Banerjee and Uday Bandyopadhyay. Biological activities and medicinal properties of neem (Azadirachtaindica), Current Science2002;82(11):1336-1345.
2. Padma TV. India Ayurveda. Nature.2005;436-486.
3. WHO guidelines on good manufacturing practices (GMP) for herbal medicines.2007:4.
4. K. Senthil Kumaran, P. Thirugnanasambantham, S. Viswanathan, M. Sree Rama Murthy. An HPLC method for the estimation of Andrographolide in rabbit serum. Indian J. Pharmacol2003; 35:109-112.
5. KotagiriRavikanth, Anil Kanaujia, Parveen Singh & Deepak Thakur. Validated RP-HPLC method for the quantification of Andrographolide in toxiroak premix, A polyherbal mycotoxin inhibitor. Int J Pharm Sci Res 2013; 4(7):2623-2628.
6. Xu W, Yang W. Assay of andrographolide bulk drug by HPLC.ZhongguoZhongyaoZazhi 2010;35(16):2113-2115.
7. Bhope S.G., Kuber V.V., Nagore D.H., Gaikwad P.S., Patil M.J. Development and validation of RP-HPLC method for simultaneous analysis of andrographolide, phyllanthin and hypophyllanthin from herbal hepatoprotective formulation. Acta Chromatogr 2013; 25(1):159-169.
8. AnubhutiPasrija, Rahul Singh, and Chandra kantKatiyar. Validated HPLC-UV method for the determination of berberine in raw herb Daruharidra (Berberis aristate DC), its extract, and in commercially marketed Ayurvedic dosage forms. Int J Ayurveda Res 2010;1(4):243-246.
9. Devaliya R, Shirsat M. Development and Validation of RP-HPLC Method for Quantification of Berberine in Ethanol Fraction of Methanol Extract and Developed Formulation of TinosporaCordifolia. Orient J Chem 2017;33(2):989-994.
10. Jing-Chun Wang, Qi Zhang, and De-Fu Cai, Stability-Indicating Validated HPLC Method for Analysis of Berberine Hydrochloride and Trimethoprim in Pharmaceutical Dosage Form, Journal of Chemistry, vol. 2013, Article ID 360812, 9 pages,2013.
11. Herbal Hills, Liv-First Tablets, Liver Support - Ayurvikalp.comfrom https://www.ayurvikalp.com/en-gb/product/herbal-hills-liv-first-tablets-liver-support
12. Joseph J and Solomon Jeeva . Andrographis paniculata: A Review of its Traditional Uses, Phytochemistry and Pharmacology. Med Aromat Plants 2014;3(4):2-14.
13. JuttiLevita, As’ari Nawawi, Abdul Mutalib and Slamet Ibrahim. Andrographolide: A review of its Anti-inflammatory activity via inhibition of NF-KappaB activation from computational chemistry aspects. Int. J. Pharmacol. 2010;6:569-576.
14. Chander V, Aswal JS, Dobhal R, Uniyal DP. A Review on Pharmacological potential of Berberine; an active component of Himalayan Berberis aristata. J Phytopharmacol 2017;6(1):53-58.
15. Sharma Komal, Bairwa Ranjan, Chauhan Neelam, Shrivastava, Birendra, Saini Neeraj Kumar. Berberis aristata: A Review; IJRAP 2011;2(2):383-388.
16. ICH harmonised, tripartite guideline validation of analytical procedures: text and methodology q2(r1), Geneva,2005:1-13.