^Back To Top
Arpita Roy and Navneeta Bharadvaja*
Plant Biotechnology Laboratory, Department of Biotechnology,
Delhi Technological University, New Delhi-110042, India
The objective of the study was to assess the effect of three plant tissue culture media (MS, Gamborg’s B5 and Nitsch) on the growth of five accessions of Centella asiatica using nodal explant. All the three media supplemented with 1 mg/l BAP resulted in increased growth. Amongst the three Medias, MS media resulted in the highest shoot multiplication in all the five accessions after eight weeks of the incubation period. The highest number of shoots and maximum shoot length were obtained in accession number 342109 (19.6±0.57 shoot number, 4.9±0.53 shoot length) grown in MS media supplemented with 1 mg/l BAP. Estimation of stigmasterol, an important phytosterol present in the leaves (main part for synthesis) was done by using gas chromatography and the highest amount of stigmasterol was obtained in accession number 342109 i.e. 0.0228µg/mg of leaves.
Keywords- Centella asiatica; MS media; B5 media; Nitsch media; Stigmasterol; Gas Chromatography
1. Arab MM, Yadollahi A , Shojaeiyan A, Shokri S, Maleki Ghojah S. (2014). Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G × N15 (hybrid of almond × peach) vegetative rootstock. Journal of Genetic Engineering and Biotechnology, Volume 12(2): 81–87
2. Chowdhary A., Chaturvedi P., and Memon R. (2014). Stigmasterol variation in a Medhya Rasayan plant (Centella asiatica L.: Apiaceae) collected from different regions. Indian Drugs, 51(03)
3. Collin H.A (2001). Secondary product formation in plant tissue cultures. Plant Growth Regulators, 34: 119-134,
4. Dj.V. Ruzic, T.I. Vujovic (2008) Hort Science. 3, 12–21.
5. Ghanti K, Kaviraj CP, Venugopal RB, Jabeen FTZ and Srinath Rao (2004). Rapid regeneration of Mentha piperita L. from shoot tip and nodal explants. Indian Journal of Biotechnology, 3, 594-598.
6. Gnanapragasam A, Yogeeta S, Subhashini R, Ebenezar KK, Sathish V, Devaki T (2007) Adriamycin induced myocardial failure in rats: Protective role of Centella asiatica. Molecular and Cellular Biochemistry 294: 55–63.
7. Heong S. Ariffin F, Kaur B, Karim AA, Huda N (2011) Antioxidant capacity and phenolic composition of fermented Centella asiatica herbal teas. Journal of the Science of Food and Agriculture 91: 2731–2739.
8. Khanna S., Roy S., Parinand N.L. Maurer i, M., Sen,C.K (2006). Characterization of thepotent neuroprotective properties of the natural vitamin E -tocotrienol, Journal of Neurochemistry, 98, 1474–1486.
9. Krishnamurthy RG, Senut MC, Zemke D, Min J, Frenkel MB, Greenberg EJ, Yu SW, Ahn N, Goudreau J, Kassab M, Panickar KS, Majid A (2009) Asiatic Acid, a Pentacyclic Triterpene from Centella asiatica, Is Neuroprotective in a Mouse Model of Focal Cerebral Ischemia. Journal of Neuroscience Research 87:2541–2550.
10. Malloy, MJ; Kane, JP (2001). A risk factor for atherosclerosis: Triglyceride-rich lipoproteins. Advances in Internal Medicine 47: 111–36.
11. Molassiotis A, Dimassi K, Therios I, Diamantidis G (2003) Biologia Plantarum, 47, 141–144.
12. Nguyen, H.L. and Huynh, V. K. (2011). Micropropagation of Solanum hainanense Hance. Annals of Biological Research., 2(2):394-398.
13. Qureshi A.A., Qureshi N. , Hasler-Rapacz J.O., Weber F.E., Chaudhary V., Crenshaw T.D., Gapor A., A.S.H. Ong, Y.H. Chong, D. Peterson, J. Rapacz, Dietarytocotrienols reduce concentrations of plasma cholesterol apolipoprotein B,thromboxane B2, and platelet factor 4 in pigs with inherited hyperlipidemias. The American Journal of Clinical Nutrition, 53 (1991) 1042S–1046S.
14. Saxena G and Flora SJS (2006) Changes in brain biogenic amines and haem biosynthesis and their response to combined administration of succimers and Centella asiatica in lead poisoned rats. Journal of Pharmacology and Pharmacotherapeutics, 58: 547–559.
15. Sivakumar G., Alagumanian S., Rao MV. (2006) High Frequency in vitro Multiplication of Centella asiatica: An Important Industrial Medicinal Herb. Eng. Life Sci, 6, No. 6, 597–601
16. Sethiya and Mishra S (2015) Simultaneous HPTLC Analysis of Ursolic Acid, Betulinic Acid, Stigmasterol and Lupeol for the Identification of Four Medicinal Plants Commonly Available in the Indian Market as . Journal of Chromatographic Science, 53 (5): 816-823.
17. Soumyanath A, Zhong Y. P, Gold S.A., X Yu, D. Koop, Bourdette D and Gold B. G. (2005). Centella asiatica acceslerates nerve regeneration upon oral administration and contains multiple active fractions increasing neurite elongation in vitro. Journal Pharmacy and Pharmacology, 57(9), 1221-9.
18. Sriramya G, Maheshwari R, Varahalarao V, Krishna Madhav N, et al., (2017). Acetylcholinesterase inhibitory activity of stigmasterol & hexacosanol is responsible for larvicidal and repellent properties of Chromolaena odorata. Biochimica et Biophysica Acta, 1861, 541–550
19. Subban R, Veerakumar A, Manimaran R, Hashim KM, Balachandran I (2008) Two new flavonoids from Centella asiatica (Linn.). Journal of Natural Medicines 62:369–373.
20. Susana Mangas , Merce Bonfill , Lidia Osuna , Elisabeth Moyano , Jaime Tortoriello , Rosa M. Cusido , M. Teresa Pin˜ ol , Javier Palazo. The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants. Phytochemistry 67 (2006) 2041–2049.
21. Tilvis, RS; Miettinen, TA (1986). "Serum plant sterols and their relation to cholesterol absorption". The American Journal of Clinical Nutrition 43 (1): 92–7.
22. Veenrendra Kumar M.H, Gupta Y.K (2002). Effect of different extracts of Centella asiatica on congnition and markers of oxidative stress in rats. Journal of Ethnopharmacology, 79, 253-60.
23. Vahouny GV, Connor WE, Subramaniam S, Lin DS, Gallo LL (1983). Comparative lymphatic absorption of sitosterol, stigmasterol, and fucosterol and differential inhibition of cholesterol absorption. The American Journal of Clinical Nutrition, 37(5):805-9.
24. Weng XX, Zhang J, Gao W, Cheng L, Shao Y, Kong DY (2012) Two New Pentacyclic Triterpenoids from Centella asiatica. Helvetica Chimica Acta –Vol. 95: 255-260.
25. Woyengo, T A; Ramprasath, V R; Jones, P J H (2009). "Anticancer effects of phytosterols". European Journal of Clinical Nutrition 63 (7): 813–20.
26. Zhang FL, Wei YJ, Zhu J, Gong ZN (2008) Simultaneous quantitation of three major triterpenoid glycosides in Centella asiatica extracts by high performance liquid chromatography with evaporative light scattering detection. Biomedical Chromatography 22:119-12