In-Vitro Cytotoxicity Evaluation Of Makardhwaja Against Mcf-7 Human Cell Lines

Published: Saturday, 13 September 2014 10:43 | Written by Super User | Print | Email | Hits: 6039

Dr. Ninad Sathe¹, Dr. Rohit Chavan², Dr. Mujahid Khan³, Dr. Ashish punde⁴

Paper Index :

¹.Professor, Dept. of Rasashastra & Bhaishajyakalpana, YMT Ayurvedic Medical College, Kharghar, Navi Mumbai,

^2,3.MD (Rasashastra & Bhaishajyakalpana)

⁴.Assistant professor, Dept. of Rasashastra & Bhaishajyakalpana, YMT Ayurvedic Medical

College, Kharghar, Navi Mumbai,

Abstract

Herbo-mineral formulations are gaining negative propaganda in population. Hence, mankind can remain deficient from the bounty of effective and efficient ayurvedic formulation like makardhawaja. It is used as a rejuvenating & aphrodisiac (rasayan and vajikaran) drug. Modern medicine evolved a method of subjecting all assumptions to experimental verification and statistical validation. Therefore, current research had been undertaken with an aim to study cytotoxicity against selected MCF-7 human cell lines. Makardhwaj was prepared according to the reference of rasachandanshu which is a herbo-mineral formulation. Its cytotoxicity study was conducted at ACTREC. The solvent used for dissolution of the study drug was DMSO. Human cancer cell lines MCF-7 of breast were cryopreserved in liquid nitrogen and DMSO at -20⁰C. Cell culture was performed in incubators at 37°C .The selected cell lines were inoculated in 96 well plates, in which study drug was tested at 4 dose levels i.e. 10, 20, 40, 80 µg/ml. Adriamycin was used as a positive control drug for comparative screening. According to SRB assay protocol Lc50values were calculated as declared by NCI. The study had revealed that, makardhwaja is not cytotoxic as the observed LC50 values were found > 80 in the study.

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Category: Volume 6 Issue 6 (Nov.-Dec. 2016)

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In-Vitro Cytotoxicity Evaluation Of Makardhwaja Against Mcf-7 Human Cell Lines

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