Effect of Snow Mountain Garlic Extracts on Cellular Count and Viability in Cell Lines Cervical Cancer

Published: Saturday, 13 September 2014 10:43 | Written by Super User | Print | Email | Hits: 3034

Zúñiga-Martínez M L¹, Terán-Figueroa Y², Vértiz-Hernández A A³, Alcántara-Quintana L E⁴

DOI : http://dx.doi.org/10.31142/ijahm/v9i4.07

¹Doctorado en Ciencias Biomédicas Básicas, Universidad Autónoma de San Luis Potosí

²Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí

³Coordinación Académica Región Altiplano, Universidad Autónoma de San Luis Potosí

⁴Cátedra CONACYT, adscrita a Facultad de Enfermería y Nutrición, Universidad Autónoma

de San Luis Potosí

*Corresponding author: Alcántara-Quintana L E

Cátedra CONACYT, adscrita a Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí

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Abstract

Introduction: Some varieties of garlic - such as the Snow Mountain variety - have been attributed a greater amount of medicinal effects than the rest of the Allium genus, due mainly to the amount of sulfur compounds they contain. The amount of these compounds depends - among other circumstances - on the extraction conditions and techniques.

Objective: To assess the effects of Snow Mountain garlic extracts, obtained by different processes, on count and cell viability in Cervicouterine Cancer cell lines.

Material and methods: Three extraction methods were used to obtain sulfur compounds; three different concentrations of each extract were prepared and their effects were evaluated on two neoplastic and one non-neoplastic cell lines. The evaluation methods were Neubauer chamber cell count at 24, 48 and 72 hours and a MTT test at 72 hours.

Results: A significant dose-dependent increase in the number and viability of neoplastic cells was observed (p = 0.0001 and p = 0.0003 respectively), specially in soxhlet extraction treatments.

Conclusion: the ethanolic, chloroformic and aqueous Snow Mountain garlic extractions show an increase in the number and viability of Cervicouterine Cancer (CuCa) cells, contrary to other Allium varieties, so their probable anti-neoplastic activity requires further study.

Keywords: cancer, Allium sativum L., Snow Mountain garlic, cell count and viability.

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Category: Volume 9 Issue 4 [July-August 2019]

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Effect of Snow Mountain Garlic Extracts on Cellular Count and Viability in Cell Lines Cervical Cancer

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1	SIMPLE	t	NULL	eq_ref	PRIMARY,tag_idx,idx_access	PRIMARY	4	rajourna_ijahm.m.tag_id	1	100.00	Using where

id	select_type	table	partitions	type	possible_keys	key	key_len	ref	rows	filtered	Extra
1	SIMPLE	a	NULL	ref	idx_state,idx_access,idx_context	idx_state	1	const	1	100.00	Using index condition; Using where; Using temporary; Using filesort
1	SIMPLE	l	NULL	eq_ref	idx_langcode	idx_langcode	28	rajourna_ijahm.a.language	1	100.00	Using where
1	SIMPLE	uc	NULL	eq_ref	PRIMARY	PRIMARY	4	rajourna_ijahm.a.checked_out	1	100.00	NULL
1	SIMPLE	ag	NULL	eq_ref	PRIMARY	PRIMARY	4	rajourna_ijahm.a.access	1	100.00	Using where
1	SIMPLE	ua	NULL	eq_ref	PRIMARY	PRIMARY	4	rajourna_ijahm.a.created_user_id	1	100.00	Using where
1	SIMPLE	g	NULL	eq_ref	PRIMARY	PRIMARY	4	rajourna_ijahm.a.group_id	1	100.00	Using where
1	SIMPLE	fc	NULL	ref	PRIMARY	PRIMARY	4	rajourna_ijahm.a.id	1	100.00	Using where; Using index; Distinct

id	select_type	table	partitions	type	possible_keys	key	key_len	ref	rows	filtered	Extra
1	SIMPLE	a	NULL	ref	idx_access,idx_state,idx_catid,idx_language	idx_catid	4	const	8	16.00	Using index condition; Using where; Using filesort
1	SIMPLE	cc	NULL	const	PRIMARY	PRIMARY	4	const	1	100.00	NULL

Status	Duration
starting	0.17 ms
checking permissions	0.00 ms
checking permissions	0.00 ms
Opening tables	0.02 ms
init	0.06 ms
System lock	0.01 ms
optimizing	0.02 ms
statistics	0.13 ms
preparing	0.04 ms
Sorting result	0.01 ms
executing	0.00 ms
Sending data	0.01 ms
Creating sort index	0.24 ms
end	0.01 ms
query end	0.01 ms
closing tables	0.03 ms
freeing items	0.04 ms
cleaning up	0.01 ms

Status	Duration
starting	0.09 ms
checking permissions	0.01 ms
Opening tables	0.03 ms
init	0.03 ms
System lock	0.01 ms
optimizing	0.01 ms
statistics	0.06 ms
preparing	0.02 ms
executing	0.00 ms
Sending data	0.11 ms
end	0.00 ms
query end	0.01 ms
closing tables	0.02 ms
freeing items	0.05 ms
cleaning up	0.02 ms

id	select_type	table	partitions	type	possible_keys	key	key_len	ref	rows	filtered	Extra
1	SIMPLE	m	NULL	ref	PRIMARY,published,newsfeeds,idx_language	published	1	const	12	3.33	Using index condition; Using where; Using filesort
1	SIMPLE	e	NULL	ref	element_clientid,element_folder_clientid	element_clientid	403	rajourna_ijahm.m.module,const	1	10.00	Using index condition; Using where
1	SIMPLE	mm	NULL	ref	PRIMARY	PRIMARY	4	rajourna_ijahm.m.id	1	40.00	Using where; Using index