Dandge P.B¹*,  Kulkarni A. S², Nadaf S. H³, Dandge P.B⁴, Shinge A. J⁵,  Rajguru S. V⁶

^1,2,3Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur (MS) India

⁴Department of Chemistry, Shivaji University, Vidyanagar,Kolhpur (MS) India.

 ^5,6Department of Statistics, Shivaji University, Vidyanagar,Kolhpur (MS) India  

http://dx.doi.org/10.47191/ijahm/v14i6.01 

Abstract:Herbal plants have long been used in traditional medicine to address and heal a wide range of health-related issues. Withania coagulans (Family; Solanaceae), a valuable medicinal plant used to evaluates bioactives by using maceration. Phytochemical analysis evaluated by qualitatively for five different extract. Methanol extract has highest phytocostitutents so further used for analysis phenolic compounds (86.67 ± 3.19 mg GAE/g) and flavonoids (60.95 ± 2.70 mg QE/g). The methanol extract demonstrated significant antioxidant activity in the DPPH and FRAP assay with IC₅₀ value 63.02±1.08 μg/ml and 70.2±1.11 μg/ml respectively and notable antibacterial activity against Escherichia coli and Staphylococcus aureus. Additionally, the methanol extract exhibited substantial anti-diabetic potential, with IC₅₀ value 602.6±2.98 μg/ml. This study demonstrated that the methanolic extract of Withania coagulans is a potent natural antioxidant, which could be beneficial in slowing the progression of various oxidative stress-related conditions.

KEYWORDS: Withania coagulans, antioxidant, anti-diabetic

REFERENCES:

1. JeyalatchaganS, Rajendran S, Muniappan An ethnopharmacological analysis of medicinal plants used by the Adiyan community in Wayanad district of Kerala, India. European journal of integrated medicine 2017; 12: 60-73
2. Sisubalan N, Velmurugan S, Malayaman V, Thirupathy S, Basha M, Ramasamy R. Ethnomedicinal studies on villages of Thenpuranadu, Tamil Nadu, India. Sapatula DD 2014; 4(1): 41-47
3. Shree Krishna M, Sandhiya V, Sankardoss N, Velayutham R. Traditional Uses, Phytochemistry and Pharmacology of Withania coagulans: A Review. Inventi Rapid: Ethanopharmacology 2013; 1: 1-6
4. Ojha S, Alkaabi J, Naheed A, Sheikh A, Agil A, Abdelmonem M,  Adem A. Withania coagulans Fruit Extract Reduces Oxidative Stress and Inflammation in Kidneys of Streptozotocin-Induced Diabetic Rats. Oxidative Medicine and Cellular Longevity 2014 ; 1-9
5. Farooq M, Naseer U, Aziz A, Zafar S, Qadir I, Farooq M,  Ahmad I, Khayyam A. Antioxidant and Phytochemical composition of Leaves, Stem and Root Extracts of Withania coagulans and Withania somnifera. Journal of Medicinal and Spice plants 2020; 24(1): 27-30
6. Rakesh Maurya R, Akanksha , Jayendra , Singh A , Srivastava  Coagulanolide, a withanolide from Withania coagulans fruits and antihyperglycemic activity. Bioorganic & Medicinal Chemistry Letters 2008; 18(24): 6534-6537
7. Agarwal N, Raghav K, Singh P. A Promising Therapeutic Agent. International Journal of Green and Herbal Chemistry. 2014; 3:701-711.
8. Khan M, Maqsood M, Saeed R, Alam A, Sahar A, Kieliszek M. Phytochemistry, Food Application, and Therapeutic Potential of the Medicinal Plant (Withania coagulans): A Review. Molecules 2021; 26(22): 6881   
9. Tambun R, Alexander V, Ginting Y. Performance comparison of maceration method, soxhletation method, and microwave-assisted extraction in extracting active compounds from soursop leaves (Annona muricata): A review. IOP Conference Series Material Science and Engineering 2021; 1122(1): 012095
10. VaradharajanV, Janarthanan U, Krishnamurthy V. Physicochemical, phytochemical screening and profiling of secondary metabolites of annonasquamosa leaf extract. World Journal of Pharmaceutical research 2012; 1(4): 1143-1164
11. Du J, Zhong B, Subbiah V, Barrow C, Dunshea F. LC-ESI-QTOF-MS/MS profiling and antioxidant activity of phenolics from custard apple fruit and by-products. Separations 2021; 8(5):2-26
12. Kalaisezhiyen P, Vadivukkarasi S. Evaluation of free radical scavenging activity of various leaf extracts from Kedrostisfoetidissima (Jacq.) Cogn. Foood science and human wellness 2015; 3(2): 1-7
13. Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autioxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural & Food Chemistry 1992; 40: 945- 948.
14. Benzie I, Strain J. Simultaneous automated measurement of total ‘antioxidant’ (reducing) capacity and ascorbic acid concentration. Redox Report communication in free radical 1997; 3(4): 233-238
15. Dakhouche D, Bennamoun S, Amel A, Kenza L, Tahar N, Zoubida G, Zahia M. An optimization study of α- amylase production by Aspergillus niger ATCC 16404 grown on orange waste powder.  Methods on Enzymology 1955; 149–158.
16. Peerzade N, Sayed N, Das N. Antimicrobial and phytochemical screening of methanolic fruit extract of Withania coagulans L. Dunal for evaluating the antidiabetic activity. The Pharma Innovation 2018; 7(1): 197-204
17. Salwaan C , Singh A , Mittal A , Singh P. Investigation of the Pharmacognostical, Phytochemical and Antioxidant Studies of Plant Withania coagulans Dunal. Journal of Pharmacognosy and Phytochemistry 2012; 1(3): 32-39
18. Mittal S, Rao N, Menghan E. Phytochemical and antimicrobial activity of Withania coagulans (stocks) Dunal (Fruit). International Journal of Phramacy and Pharmaceutical Sicences 2012;4(4):387-389
19. Hsiu J, FischerH Stein E. Alpha-Amylases as Calcium-Metalloenzymes. II. Calcium and the Catalytic Activity. Biochemistry 1964; 3: 61–66
20. Alqahtani A. Hidayathulla S, Rehman M, ElGamal A, Massarani S et al. Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia. Biomolecules 2019; 10(1): 61
21. Kaur N, Sharma S. Quantitative Estimation of Phytoconstituents, Antioxidants and in vitro Antidiabetic Activity of Polyherbal Extract. Journal of Young Pharamacists 2022; 14(4): 382-386

Dr. Sabarinath M K

Assistant Professor, Dept. of Shalakya tantra, SDM College of Ayurveda, Hospital and Research Centre Udupi.

http://dx.doi.org/10.47191/ijahm/v14i6.02

ABSTRACT:

Introduction: Indralupta is one among the shiro kapalagata roga. According to Acharya Vagbhatta, pitta along with vata invades the romakoopa causes hairfall. Vitiation of Sleshma along with shonita obstructs the channels of Romakoopa leading to the stoppage of regrowth of hair¹. This condition is known as Indralupta. Indralupta can be correlated with Alopecia areata in which there is a sudden hairfall that strarts with one or more circular bald patches. In Ayurveda both shodhana and shamana treatments are prescribed for Indralupta. This is a case report of 25 year old male patient complaints of patchy hair loss and thinning of hair since 2 months.

Materials and Methods: The subject who approached Shalakya tantra opd of SDM Ayurveda hospital Udupi with symptoms of patchy hair loss and thinning of hair since 2 months was systematically reviewed and treatment modalities like pracchana and internally Arogyavardhini rasa, Tiktaka kashaya, Saptamruta loha and Bhargava prokta rasayana is advised.

Results: The subject showed marked improvement symptomatically.

Discussion: Ayurveda has great approach in curing the Indralupta. Nidana parivarjana, shamanoushadis and external applications are the main treatment modalities which can be concluded from this case report.

KEYWORD :Indralupta, Alopecia areata, Pracchana

REFERENCES:

1. Acharya vagbhatta: Ashtanga hrudaya text with English translation by prof. K.R Srikantha murthy. Varanasi: choukhamba krishnadas academy: reprint edition 2006. Uttara tantra, chapter 23, shloka 24-25, page 222.
2. Agnivesha: Charaka samhita with Ayurveda deepika commentary edited by Vaidya Jadavaji Trikamji Acharya, Varanasi: choukhambha prakashan: reprint edition 2009. Chikitsa sthana,chapter 15, shloka 96-97, page 520.
3. Acharya vagbhatta: Ashtanga hrudaya text with English translation by prof. K.R Srikantha murthy. Varanasi: choukhamba krishnadas academy: reprint edition 2006. Uttara tantra, chapter 24, shloka 24, page 228.
4. Acharya vagbhatta: Ashtanga hrudaya text with English translation by prof. K.R Srikantha murthy. Varanasi: choukhamba krishnadas academy: reprint edition 2006. Uttara tantra, chapter 39, shloka 169-173, page 410.
5. Agnivesha: Charaka samhita with Ayurveda deepika commentary edited by Vaidya Jadavaji Trikamji Acharya, Varanasi: choukhambha prakashan: reprint edition 2009. Chikitsa sthana,chapter 15, shloka 19, page 515.

 Shehu M.M.^1,2*, Vantsawa, P.A.², Appah, J.², Dan, V.M.Y.², Muhammad J.³, Bello N.K.⁴, Abubakar N.⁵

¹Department of Biological Sciences, Federal University Gashua, PMB 1005, Yobe, Nigeria

²Department of Biological Sciences, Nigerian Defence Academy, PMB 2109, Kaduna, Nigeria

³Department of Human Physiology, Federal University Birnin kebbi, PMB 1157, Kebbi, Nigeria

⁴Department of Human Anatomy, Federal University Birnin kebbi, PMB 1157, Kebbi, Nigeria

⁵Department of Pathology, Federal Medical Center Gusau PMB 1001, Zamfara, Nigeria

http://dx.doi.org/10.47191/ijahm/v14i6.03

ABSTRACT:

African Animal Trypanosomiasis constitutes one of the greatest threats to the health of animals and socioeconomic status of people, particularly in developing countries. Chemotherapy, the main means of controlling the disease is limited due to parasite resistance and toxicity of the current anti-trypanosomal drugs. The development of a vaccine has been thwarted by antigenic variation of the parasite. Thus, plant extracts are one of the strategies being explored to address some of the problems encountered. The main objective of the current study was to investigate the toxicity and anti-trypanosomal activity of methanol and aqueous extracts of Acacia nilotica through in vivo assays against Trypanosoma brucei brucei. The chosen plants' healthy, fresh, matured leaves were air dried under shade, pulverized with mortar and pestle into powder, and passed through a 0.5mm mesh to standardize their particles. The plant samples were extracted using aqueous, methanol solvents. Qualitative and quantitative phytochemical analysis of the active chemical constituents of the extracts was conducted to determine saponins, flavonoids, tannins, terpenoids, steroids, and cardiac glycosides according to standard procedure. It was established that aqueous and methanol leaf extracts of the selected plants were safe in rats at dose levels of 1000, 3000, and 5000 mg/kg body weight for 72 hours. However, Sedation and abnormal movement were observed for both A. nilotica and Z. mucronata as manifestations of clinical toxicity at 5000mg/kg body weight. The fatal dose of all extracts exceeds the maximum dose of 5000mg/kg. Prolonged oral administration of the extracts for 21 days with A. nilotica extracts did not reveal major changes in body and organs weights, liver and kidney functions, the biochemical analysis showed a slight, non-significant increase in Alanine transaminase, Aspatate transaminase, and Alkaline phosphatase at (4000mg/kg) in rats, the average creatinine and urea levels were within the normal range, the relative organ weight and isolated organ are within the normal reference value. The photomicrographs of the liver and kidney sections showed mild histological changes. The in vivo assay showed the aqueous and methanol extracts from A. nilotica at 200mg/kg, 100mg/kg, and positive control (diminazine aceturate 3.5mg) reduced parasitaemia (p < 0.05), improved anaemia (p < 0.05), prevented body weight loss (p < 0.05) compared to the negative control. This study showed that the leaf of A. nilotica is safe and possess antitrypanosomal properties, suggesting that they may be a source of novel drugs for treatment of tropical diseases caused by trypanosomes.

KEYWORD :Acacia nilotica, Trypanosomiasis, Toxicity, Histopathology, Trypanasoma brucie brucei

REFERENCES:

1. D’Archivio S, Medina M, Cosson A, Chamond N, Rotureau B, Minoprio P, et al. Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. PLoS Negl Trop Dis. 2011;5(12): e1461. https://doi.org/10.1371/journal.pntd.0001461.
2. Cayla M, Rojas F, Silvester E, Venter F, Mathews KR. African trypanosomes. Parasit. Vectors. 2019;12:190. https://doi.org/10.1186/s13071-019-3355-5.
3. WHO. interim guidelines for the treatment of gambiense human African trypanosomiasis. Geneva: World Health Organization; 2019. Licence: CC BY-NC-SA 3.0 IGO. Available from: https://www.ncbi.nlm. nih.gov/books/NBK545514/
4. Anita RE, Olayemi JO, Aina OO, Ajaiyeoba EO. In vitro and in vivo animal model antitrypanosomal evaluation of ten medicinal plant extracts from southwest Nigeria. Afr J Biotechnol. 2009;8(7):1437–40.
5. Bizimana N, Tiejen U, Zessin KH, Diallo D, Djibril D, Melzig MF, et al. Evaluation of medicinal plants from Mali for their in-vitro and in-vivo trypanocidal activity. J Ethnopharmacol. 2006;103:350–6.
6. Holmes P. Tsetse-transmitted trypanosomes – their biology, disease impact and control. J. Invertebr Pathol. 2013;112 Suppl:S11–4.
7. Swallow BM. Impacts of Trypanosomiasis on African Agriculture. In: PAAT Technical and Scientific Series No. 2. Rome, Italy: Food and Agriculture Organisation of the United Nations (FAO); 2000.
8. Toya NB. Immunobiology of African trypanosomes: Need of alternativeinterventions. J Biomed Biotechnol. 2010;2010:389153. https://doi.org/10.11 55/2010/389153.
9. Barrett MP, Vincent IM, Burchmore RJ, Kazibwe AJ, Matovu E. Drug resistance in human African trypanosomiasis. Future Microbiol. 2011;6(9): 1037–47.
10. Wurochekke AU, Anyanwu GO. Antitrypanosomal activity of anogeissus leiocarpus in rats infected with Trypanosoma brucei brucei. Int Res J Biotechnol. 2012;3(1):5–9
11. Nwodo, N., Okoye, F., Lai, D., Debbab, A., Kaiser, M., Brun, R., & Proksch, P. (2015). Evaluation of the in vitro trypanocidal activity of methylated flavonoid constituents of Vitex simplicifolia BMC Complementary Alternative Medicine. 15:82. doi: 10.1186/s12906-015-0562-2.
12. Abimbola, A. M., Baba, I. A., Yenusa, E. Z., Omanibe, S. J., & Oladimeji, I. H. (2013). Anti-trypanosomal effect of Peristrophe bicalyculata extract on Trypanosoma brucei brucei-infected rats. Asian Journal of Tropical Biomedicine 7: 523–31.
13. Omale, J., & Omajali, B. (2010). Studies on some nutritional characteristics of the fruit and leaf of Saba florida (Benth) from Ibaji forest. International Journal of Nutrition and Metabolism 2: 12–27.
14. Atawodi, S. E., Bulus, T., Ibrahim, S., Ameh, A., Nok, A.J., & Mamman, M. (2003) In vitro trypanocidal effect of methanolic extract of Nigerian savannah plants. African Journal of Biotechnology (9): 31–21.
15. Onotu, C. S, Musa U. B., Fajinmi, A. O, & Shaida, S. S. (2013). Physiochemical evaluation of ethanolic root extract of Carissa spinarum (Wild Karanda) on Trypanosoma brucei brucei (Federe Strain) infected mice. International Journal of Pharmaceutical Science Invention. 2:18–26.
16. Tauheed, A. M., Shittu, S. H., Suleiman, M. M., Habibu, B., Kawu, M. U., & Kobo, P. I. (2016). In vivoameliorative effects of methanol leaf extract of Lawsonia inermis Linn on experimental Trypanosoma congolense infection in Wistar rats. International Journal of Veterinary Science and Medicine. 4:33–40. doi: 10.1016/j.ijvsm.2016.10.005. 
17. Tauheed, A. M., Mamman, M., Ahmed, A., Suleiman, M. M. & Balogun, E. O. (2020). In vitro and in vivo antitrypanosomal efficacy of combination therapy of Anogeissus leiocarpa, Khaya senegalensis and potash. Journal of Ethnopharmacology. 258, 112805.
18. Rufa'i, F.A., Baecker, D., & Mukhtar, M. D. (2020). Phytochemical Screening, GC-MS Analysis, and Evaluating In Vivo Antitrypanosomal Effects of a Methanolic Extract of Garcinia kolaNuts on Rats. Antibiotics (Basel). 12(4):713. doi: 10.3390/antibiotics12040713.
19. Sirak, B., Bizuneh, G. K., Imming, P., & Asres, K. (2024). In vitro and in vivo antitrypanosomal activity of the fresh leaves of Ranunculus Multifidus Forsk and its major compound anemonin against Trypanosoma congolense field isolate. BMC Veterinary Research. 20(1):32. doi: 10.1186/s12917-023-03856-1.
20. Rather, L., Mohammad, F. Luka, J., & Bushar, K (2015). Acacia nilotica (L.): A review of its traditional uses, phytochemistry, and pharmacology. Sustain Chemical Pharmacology 2:12–30.
21. Taresa, B. B. (2018). Extraction and quantitative phytochemical screening of medicinal plant: a brief summary. International Journal of Pharmacy. 8(1):137-143.
22. Organization for Economic Co-operation and Development. OECD guidelines for the testing of chemicals. Paris: Organization for Economic Co-operation and Development; 2002. [Online] Available from: http://www.oecd.org/chemicalsafety/testing/2741541. pdf [Accessed on 27th May, 2023]
23. Kifayatullah, M., Mohd, S. M., Pinaki, S., Moklesur, R. S., & Arindam. D. (2015). Evaluation of the acute and sub-acute toxicity of the ethanolic extract of Pericampylus glaucus (Lam.) Merr. in BALB/c mice. Journal of Acute Diseasem 4(4) 309-315
24. Chechet, G. D., Yahaya, J., & Nok, A. J. (2018). In vitro and in vivo Anti-trypanosomal Potentials of Afrormosia laxiflora and Khaya seegalensis against Trypanosoma brucei brucei. Nigerian veterinary journal (3): 269-284
25. Owolarafe, T. A., Fadilu, M., Salawu, K., Ononamadu, C. J., Lawal, A. T., Ihegboro, G. O., Barau, M. M. (2017). Effect of Aqueous Extract of Senna occidentalis leaves on Haematological and liver Biochemical Parameters in Wistar Rats International Journal of Pharmaceutical Sciences 6 1-8.
26. Pieme, C. A., Penlap, V.N., Nkegoum, B., Taziebou, C. L., Tekwu, E. M., Etoa, F. X., & Ngongang, J. (2006). Evaluation of acute and subacute toxicities of aqueous ethanolic extract of leaves of Senna alata (L.) Roxb (Ceasalpiniaceae). African Journal of Biotechnology. 26 (3) 283-289.
27. Aniagu, S. O., Nwinyi, F. C., Akumka, D. D., Ajoku, G. A., Dzarma, S., Izebe, K. S., Ditse, M., Patrick, E., Nwaneri, C., Wambebe, C, & Gamanie, K. (2005). Toxicity studies in rats fed nature cure bitters. African Journal of Biotechnology (4):72-78.
28. Jn, N., Charles, T., & Jurn, S, M. (2019). Acute and Sub-Chronic Toxicity Evaluation of Triplotaxis stellulifera Hutch and Crasssocephalum bougheyanum C.D. Adams Methanol Extract on Mice. Biochemistry & Analytical Biochemistry, (3): 1–10.
29. Zainal, Z., Ong, A., May, C. Y., Chang, S. K., Rahim, A. A., & Khaza’ai, H. (2020). Acute and sub chronic oral toxicity of Ziziphus mucronata in rats. International Journal of Environmental Research and Public Health, 17 (10): 56- 62
30. Herbert, W. J., & Lumsden, W. H. (1976). Trypanosoma brucei: a rapid "matching" method for estimating the host's parasitemia. Experimental Parasitology. 40(3):427-31. doi: 10.1016/0014-4894(76)90110-7. PMID: 976425.
31. Obi, C. F., Nzeakor, T. A., Okpala, M. I, Ezeh, I. O., Nwobi, L. G., Omeje, M. O, & Ezeokonkwo, R. C. (2019). Evaluation of antitrypanosomal activity of Pterocarpus santalinoides L'H'erit ex DC hydroethanol leaf extract in rats experimentally infected with Trypanosoma brucei. Journal of Ethnopharmacology. 243:112085. doi: 10.1016/j.jep..112085.
32. Ochei, J., & Kolhatkhar, A. (2000). Medical laboratory science, theory and practice. Mac Graw–hill 283.
33. Omoja, V. U., Anaga, A. O., Obidike, I. R., Ihedioha, T. E., Umeakuana, P. U., Mhomga, L. I., Asuzu, I. U., Anika, S. M. (2011). The effects of combination of methanolic leaf extract of Azadirachta indica and diminazene diaceturate in the treatment of experimental Trypanosoma brucei brucei infection in rats. Asian Pacific Journal of Tropical Medicine 4(5):337-41. doi: 10.1016/S1995-7645(11)60099-0. Epub 2011 Jun 22. PMID: 21771672.
34. Abimbola, A. M., Baba, I. A., Yenusa, E. Z., Omanibe, S. J., & Oladimeji, I. H. (2013). Anti-trypanosomal effect of Peristrophe bicalyculata extract on Trypanosoma brucei brucei-infected rats. Asian Journal of Tropical Biomedicine 7: 523–31.
35. Rasool, A., Bhat, K. M., Shiekh, A. A., Jan, A., & Hassan, S. (2020). “Medicinal Plants: Role, distribution and future,” Journal of pharmacognocy and phytochemistry, 9(2): 49-56.
36. Bisso, B. N., Nkwelle, R. N. P., Tchuenteu, R. T., and Dzoyem, J. P. (2022). Phytochemical Screening and antioxidant and antimicrobial activities of Seven Under investigated Medicinal Plants against Microbial Pathogens. Advances in Pharmacological and Pharmaceutical sciences. https://doi.org/10.1155/2022/1998808.
37. Gabi, B., Sharif, H., Umar, H., & Isyaku U. (2022). Toxicity study and effect of the leaf extract of Acacia nilotica on biochemical parameters of Wistar albino rats. Science World Journal. 3: 390-396
38. Jena, R., Rath, D., Rout, S. S., Kar, D. M. (2020). A review on genus Millettia: Traditional uses, phytochemicals, and pharmacological activities. Saudi Journal of Pharmacy 28(12):1686-1703. doi: 10.1016/j.jsps.2020.10.015
39. Prasanth, K. M., Suba, V., Ramireddy, B., & Srinivasa, B. P. (2015). Acute and subchronic oral toxicity assessment of the ethanolic extract of the root of oncoba spinosa (flacourtiaceae) in rodents. Tropical Journal of Pharmaceutical Research, 14(10) 76- 84.
40. Jain, N., Sharma, M. K., & Kaushik, P. (2022). Therapeutically Important Bioactive Compounds from Fungal Origin. Therapeutic Implications of Natural Bioactive Compounds, 3:223-235.
41. Pradhan, S., & Dubey, R. C. (2022). Deciphering antimicrobial, phytochemical, GC–MS and pharmacokinetic properties of Camellia sinensis from high-altitude region. Vegetos, 1-8.
42. Ahuja, A., Gupta, J., & Gupta, R. (2021). Miracles of herbal phytomedicines in treatment of skin disorders: natural healthcare perspective. Infectious Disorders-Drug Targets (Formerly Current Drug Targets-Infectious Disorders), 21(3), 328-338.
43. Raju, R., Prakash, T., Rahul, R., Poonangadu, S. S., Kumar, S. S., Sonaimuthu, P., & Capili, J. T. (2021). Phytochemical Analysis of Three Common Medicinal Plants (Gliricidia sepium, Melothria pendula, and Pithecellobium dulce) in the Philippines. School Academic Journal of Bioscience, 3:84-88.
44. Rodríguez-Usaquén, A., Sutachan, J. J., Villarreal, W., Costa, G. M., Acero Mondragon, E. J., Ballesteros-Ramírez R., Albarracín S. L. (2023). Sub-acute toxicity evaluation of aqueous leaf extract from Passiflora edulis Sims f. edulis(Gulupa) in Wistar rats. Toxicology Reports. 11:396-404. doi: 10.1016/j.toxrep..10.013.
45. Clarke, E. G. C. & Clarke, M. L. (1977). Veterinary Toxicology. New York: Pp 34-43
46. Umaru, B., Onyeyili P. A., & Saka, S. (2018). Anti-diarrhoeic and Antibacterial effects of aqueous and methanolic leaf extract of mucronata in rats. Nigerian Veterinary Journal, 35, 60-67
47. Taldaev, A., Terekhov, R., Nikitin, I., Zhevlakova, A., & Selivanova, I. (2022). Insights into the Pharmacological Effects of Flavonoids: The Systematic Review of Computer Modeling. International Journal of Molecular Science. 23(11):6023. doi: 10.3390/ijms23116023.
48. Yang, X., Zhang, H., Li, L., Zhou, X., Liu, Y., & Lai, J. (2022). Proteomic Analysis of Protective Effects of EpimediumFlavonoids against Ethanol-Induced Toxicity in Retinoic Acid-Treated SH-SY5Y Cells. Molecules. 27(3):1026. doi: 10.3390/molecules27031026.
49. Wang, M., Cai, X. F., Zhang, S. M., Xia, S. Y., Du, W. H., & Ma, Y. L. (2021). Alprostadil alleviates liver injury in septic rats via TLR4/NF-κB pathway. European Review for Medical and Pharmaceutical Science. 25(3):1592-1599. doi: 10.26355/eurrev_202102_24869.
50. Bellin, M. F., Valente, C., Bekdache, O., Maxwell, F., Balasa, C., Savignac, A., & Meyrignac, O. (2024). Update on Renal Cell Carcinoma Diagnosis with Novel Imaging Approaches. Cancers (Basel). 16(10):1926. doi: 10.3390/cancers16101926.
51. Kaneko, J.J. (1989) Clinical Biochemistry of Domestic animals, Academic press, San Diego. 496-537.
52. Wu, M., Ma, Y., Chen, X., Liang, N., Qu, S., & Chen, H. (2021). Hyperuricemia causes kidney damage by promoting autophagy and NLRP3-mediated inflammation in rats with urate oxidase deficiency. Disease Model and Mechanism. 14(3): 48041. doi: 10.1242/dmm.048041.
53. Juretić, N., Sepúlveda, R., D'Espessailles, A., Vera, D.B., Cadagan, C., de Miguel, M., González-Mañán, D., & Tapia, G. (2021). Dietary alpha- and gamma-tocopherol (1:5 ratio) supplementation attenuates adipose tissue expansion, hepatic steatosis, and expression of inflammatory markers in a high-fat-diet-fed murine model. Nutrition. 85:111139. doi: 10.1016/j.nut.2021.111139. Epub 2021 Jan 7. PMID: 33549947.
54. Wagner, V. A., Holl, K. L., Clark, K. C., Reho, J. J., Dwinell, M. R., Lehmler, H. J., Raff, H., Grobe, J. L., & Kwitek, A. E. (2023). Genetic background in the rat affects endocrine and metabolic outcomes of bisphenol F exposure. Toxicological Science. 194(1):84-100. doi: 10.1093/toxsci/kfad046.
55. Alli, L. A., Adesokan, A. A., Salawu, O. A., & Akanji, M. A. (2015). Toxicological studies of aqueous extract of Acacia nilotica root. Interdisciplinary Toxicology. 8(1):48-54.
56. Abebe, M. S. (2023). Acute and Subacute Toxicity of Rhamnus prinoidesLeaves on Histopathology of Liver, Kidney, and Brain Tissues, and Biochemical Profile of Rats. Journal of Toxicology. 3(2): 5615. doi: 10.1155/2023/3105615.
57. Murwanti, R., Nurrochmad, A., Gani, A. P. (2023). Acute and subchronic oral toxicity evaluation of herbal formulation: piper crocatum ruiz and pav., Typhonium flagelliforme (lodd.) blume, and Phyllanthus niruri L. In sprague-dawley rats. Journal of Toxicology.  doi: 10.1155/2023/7511397.7511397
58. Nok AJ (2002). Azaanthraquinone inhibits respiration and in vitro growth of long slender bloodstream forms of Trypanosoma congolense. Cell Biochem. Func. 20: 205–212.
59. Nwodo, N., Okoye, F., Lai, D., Debbab, A., Kaiser, M., Brun, R., & Proksch, P. (2015). Evaluation of the in vitro trypanocidal activity of methylated flavonoid constituents of Vitex simplicifolia BMC Complementary Alternative Medicine. 15:82. doi: 10.1186/s12906-015-0562-2.
60. Gayesa, R. T., Mengistu, D., & Moorhead, C. (2019). Biological activities of flavonoids: an overview. International Journal of Pharmaceutical Science and. Research.10(4):1567–1574. doi: 10.13040/ijpsr.0975-8232
61. Ullah, A., Munir, S., Badshah, S. L., Khan, N., Ghani, L., Poulson, B. G., Emwas, A. H., & Jaremko, M. (2020). Important Flavonoids and Their Role as a Therapeutic Agent. Molecules. 25(22): 5243. doi:10.3390/molecules25225243.
62. Mahmoud, A.B., Danton, O., Kaiser, M., Khalid, S., Hamburger, M., Mäser, P. (2020). HPLC-based activity profiling for antiprotozoal compounds in Croton gratissimus and cuscuta hyalina. Frontiers in Pharmacology 11:1246. doi: 10.3389/FPHAR.2020.01246.
63. Memariani, H., Memariani, M., & Ghasemian, A. (2024). Quercetin as a Promising Antiprotozoan Phytochemical: Current Knowledge and Future Research Avenues. Biomedical Research International. 7632408. doi: 10.1155/2024/7632408.
64. Adams, L., Obiri-Yeboah, D., Afiadenyo, M., Hamidu, S., Aning, A., Ehun, E., Shiels, K., Joshi, A., Mamfe Sakyimah, M., Asamoah Kusi, K., Ayi, I., Mckeon Bennett, M., & Moane, S. (2024). An In vitroand in silico investigation of the antitrypanosomal activities of the stem bark extracts of Anopyxis klaineana (Pierre) Heliyon. 10(6) 282. doi: 10.1016/j.heliyon.2024.e28025.
65. Rufa'i, F.A., Baecker, D., & Mukhtar, M. D. (2020). Phytochemical Screening, GC-MS Analysis, and Evaluating In Vivo Antitrypanosomal Effects of a Methanolic Extract of Garcinia kolaNuts on Rats. Antibiotics (Basel). 12(4):713. doi: 10.3390/antibiotics12040713.
66. Tauheed, A. M., Mamman, M., Ahmed, A., Suleiman, M. M. & Balogun, E. O. (2022). Antitrypanosomal properties of Anogeissus leiocarpaextracts and their inhibitory effect on trypanosome alternative oxidase. Phytomedicine Plus. 2(2):100223. doi: 10.1016/j.phyplu.2022.100223.
67. Tadesse, B., Terefe, G., Kebede, N., & Shibeshi. W. (2015). In Vivo anti-trypanosomal activity of dichloromethane and methanol crude leaf extracts of Dovyalis abyssinica (Salicaceae) against Trypanosoma congolense. BMC Complement Alternative Medicine. 15:278. doi: 10.1186/s12906-015-0809-y.
68. Tauheed, A. M., Mamman, M., Ahmed, A., Suleiman, M. M. & Balogun, E. O. (2022). Partially Purified Leaf Fractions of Azadirachta indica Inhibit Trypanosome Alternative Oxidase and Exert Antitrypanosomal Effects on Trypanosoma congolense. Acta Parasitologica. 67(1):120-129. doi: 10.1007/s11686-021-00437-w
69. Feyera, T., Terefe, G., Shibeshi, W. (2014). Evaluation of in vivo antitrypanosomal activity of crude extracts of Artemisia abyssinica against aTrypanosoma congolense isolate. BMC Complement Alternative Medicine. 14:117. doi: 10.1186/1472-6882-14-117.

 Dr. Rashmita Tiga¹, Prof. (Dr.) Pragya P. Mallik², Dr. Snehamayee Mishra³, Dr. Bharatilata Acharya⁴

¹MD Scholar,P.G Department of Kayachikitsa ,Gopabandhu Ayurveda Mahavidyalaya, Puri, Odisha.

²Professor & HOD, P.G Department of Kayachikitsa, Gopabandhu Ayurveda Mahavidyalaya, Puri, Odisha.

³HOD, P.G Department of Kayachikitsa, National College of Ayurveda, Barwala, Hisar, Haryana.

⁴Assistant Professor, P.G Department of Kayachikitsa, Gopabandhu Ayurveda Mahavidyalaya, Puri, Odisha.

http://dx.doi.org/10.47191/ijahm/v14i6.04

ABSTRACT:In Ayurveda, the treatment of Chronic Kidney Disease (CKD) is approached through a holistic lens, where the focus lies not just on alleviating symptoms but also on restoring balance to the body’s intricate systems. Pashanabhedadi Kwatha, a revered polyherbal formulation, exemplifies this Ayurvedic approach by combining a unique blend of herbs (such as Pashanabheda, Yastimadhu, Vasa, Gokshura, Eranda, Aragwadha phala majja, Pippali, Ela, Shilajit, Souvarchala lavana, and Mishri), each chosen for its specific role in supporting kidney health, also known for their diuretic, anti-inflammatory, and detoxifying properties. When combined, they work synergistically to enhance renal function, regulate fluid balance, and reduce inflammation, which are crucial factors in managing CKD. In Ayurveda, kidney dysfunction is often attributed to imbalances in the doshas, accumulation of ama and disturbances in agni and srotas. Pashanabhedadi Kwatha is formulated to address these imbalances, with its herbs targeting specific physiological processes that support renal and metabolic health. The present study aims to evaluate the effects of Pashanabhedadi Kwatha on CKD through the lens of Ayurvedic principles like Rasa Panchaka, and to explore its influence on doshic imbalances, ama, agni, srotas, and the specific phytochemical constituents responsible for its therapeutic benefits. By reviewing both classical and modern literature, this study seeks to shed light on the potential mechanisms behind its beneficial effects in CKD and offer insights into its clinical applications.

KEYWORD :Ayurveda, Chronic Kidney Disease, Pashanabhedadi Kwatha, Rasa Panchaka.

REFERENCES:

1. Mishra Siddhi Nandan,Bhaisajya Ratnavali of Kaviraj Govind Das Sen,Ch-34/25, Chaukhamba Surbharti Prakashan,Varanasi.
2. Pashanabheda: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:754-75; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 650-652.
3. Yastimadhu: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:152-156; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 253-256, API Vol-1, Pg no:215-217.
4. Vasa: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:407-410; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 241-244, API Vol-1, Pg no:207-208.
5. Gokshura: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:98-102; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 632-634, API Vol-1, Pg no:64-66.
6. Eranda: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:483-487; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 58-62, API Vol-1, Pg no:58-60.
7. Aragwadha: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:200-204; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 170-173, API Vol-1,Pg no:12-14.
8. Pippali: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:452-458; D.G.V by Prof. P.V Sharma Vol-2, Pg no: 275-279, API Vol-4.
9. Shilajit: Shastri Kashinath Pt.,Chaturvedi Gorakha Natha, Chaukhamba Bharati Academy,Varanasi,Vidyotini Hindi Commentary, Charak Samhita Chikitsa Sthana Ch-1.3.
10. Ela: D.G.V by Dr. J.L.N Shastry Vol-2, Pg no:527-530; D.G.V by Prof. P.V Sharma Vol-2, Pg no:719-721.
11. Souvarchala lavana: D.G.V by Dr. J.L.N Shastry Vol-3, Pg no:79; D.G.V by Prof. P.V Sharma Vol-3, Pg no: 650-652.
12. Chitranjan Das et al. A Literary Review and Therapeutic Action of Pashanbheda, JAIMS,Vol-7, July 2022.
13. Gurav & Gurav, IJPSR, 2014; Vol. 5(5): 1630-1642.
14. Damle Monica, International Journal of Herbal Medicine 2014; 2(2): 132-136.
15. Ambreen Shoaib / An ethnobotanical review of Adhatoda vasica, 2021, 67(4): 248-263.
16. Chhatre S, Nesari T, Somani G, Kanchan D, Sathaye S. Phytopharmacological overview of Tribulus terrestris. Phcog Rev 2014;8:45‑51.
17. Asian Pacific Journal of Tropical Medicine 11(3):p 177-185, March 2018.
18. Rahmani AH. Cassia fistula Linn: Potential candidate in the health management. Phcog Res 2015;7:217-24.
19. IJPSRR, Volume 5, Issue 1, November – December 2010.
20. R. Kiran et al., Review; Chemical Characteristics and Therapeutic Potential of Asphaltum Punjabianum (Shilajit),2019.
21. Kumar and Kumar, IJPSR, 2021; Vol. 12(8): 4122-4131.
22. Mestry et al., World Journal of Pharmaceutical Research, 2024.
23. Richet and M. Martin, Med. Record. XXI, 394

 Dr. Shivani¹, Prof. (Dr) Madhavi Goswami²

¹M.D. (Ayu.), Ph.D Scholar - PG Dept of Rachana Sharir, Rhishikul Campus, Uttrakhand Ayurved University.

²Former Registrar (UAU) M.D.(Ayu.), Ph.D (Ayu), PG Dept. of Rachana Sharir, Rhishikul Campus, Uttrakhand Ayurved University.

http://dx.doi.org/10.47191/ijahm/v14i6.05

ABSTRACT: Ancient acharya of ayurveda has given great emphasize on intake of aahara-ras as best source for nourishment that play prime role in growth and development of embryo/foetus during pregnancy. To achieve a healthy progeny, ayurvedic science have illuminated wholesome regimen, that should be follow by the parents before and after conception. They have also elucidate about six specific procreating factors- Shad bhav (matruja, pitruja, atmaja, rasaja, satmyaja and satwaja) which are responsible for the growth of embryo. Among six factors rasaja bhava plays an important role in providing nutrition to the foetus. The concept of Upasneha, Upasweda, Ambu, Apara and Nabhinadi are involved in nourishment of embryo/foetus at different stages of development. Anceint science have drawn attention to certain food habits (aahar ras vikriti), to be avoided by the pregnant woman to refrain from abnormalities, deformities in the offspring. The concept of nutrition (aahar ras-rasaj bhav) in garbhini explained in  ancient classics

KEYWORD : Aahar-ras, Shadbhav, Garbhaj-vikriti.

REFERENCES:

1. Charaka. Sutra Sthana, Cha.11 Tisraishaneeya Adhyaya verse 35. In: Jadavaji Trikamji Aacharya, Editor. Charak Samhita. Varanasi: Chaukhambha orientalia; 2009. p.74.
2. Charaka. Shareera Sthana, Cha.3 Khuddika garbhavakranti adhyaya verse 3. In: Jadavaji Trikamji Aacharya, Editor. Charak Samhita. Varanasi: Chaukhambha orientalia; 2009. p.308.
3. Charaka. Shareera Sthana, Cha.3 Khuddika garbhavakranti adhyaya verse 12. In: Jadavaji Trikamji Aacharya, Editor. Charak Samhita. Varanasi: Chaukhambha orientalia; 2009. p.312.
4. Sushruta. Shareera Sthana, Cha.3 Garbhavakranti adhyaya verse 33. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.354.
5. Sushruta. Sootra Sthana, Cha.14 Shonita varnaneeya adhyaya verse 3. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.59.
6. Sushruta. Sootra Sthana, Cha.14 Shonita varnaneeya adhyaya verse 16. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.63.
7. Sushruta. Sootra Sthana, Cha.14 Shonita varnaneeya adhyaya verse 3. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.59.
8. Chakrapani, Charak. chikitsa Sthana, Cha.15 Grahanidosha Adhyaya ver. 16. In: Jadavaji Trikamji Aacharya, Editor. Charak Samhita. Varanasi: Chaukhambha Orientalia; 2002. p.514.
9. Dalhana, Sushruta. Sutra Sthana, Cha. 2 Shukra shonitashuddhi adhyaya verse 33. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.348
10. Vagbhata. Sutra Sthana, Cha.11 Doshadivijnaniya Adhyaya verse 4. In: Bhisagacharya harishastri paradakara vaidya, Editors. Ashtanga Hridayam. 9th ed. Varanasi: Chaukhambha Orientalia; 2005. p. 183.
11. Sushruta. Shareera Sthana, Cha.3 Garbhavakranti adhyaya verse 31. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.353.
12. Sushruta. Shareera Sthana, Cha.3 Garbhavakranti adhyaya verse 31. In: Jadavaji Trikamji Aacharya, Narayana ram acharya, Editors. Sushruta Samhita. 7th ed. Varanasi: Chaukhambha Orientalia; 2002. p.353.
13. C.J.P. Jones, R.H. Choudhury, J.D. Aplin, Tracking nutrient transfer at the human maternofetal interface from 4 weeks to term, Placenta, Volume 36, Issue 4, 2015, Pages 372-380, ISSN-0143-4004,-https://doi.org/10.1016/j.placenta.2015.01.002; (https://www.sciencedirect.com/science/article/ pii/S0143400415000326)
14. Chakrapani, Charak. chikitsa Sthana, Cha.15 grahanidosha Adhyaya ver.16. In: Jadavaji Trikamji Aacharya, Editor. Charak Samhita. Varanasi: Chaukhambha Orientalia; 2002. p.514.
15. Charaka. Shareera Sthana, Cha.5 Shareeravichaya adhyaya verse 23. In: Jadavaji Trikamji Aacharya, Editor. Charak Samhita. Varanasi: Chaukhambha orientalia; 2009. p.334.
16. Wendy Chung. Teratogens and their effects http://www.columbia.edu/itc/hs/medical/huma ndev/2004/Chpt23-Teratogens.pdf
17. DC Dutta's Textbook of Obstetrics; Dutta, D.C. and Konar; 2015; Jaypee Brothers Medical Publisher. Pg. 47
18. Georgios V, Angeliki T, Olga T, Maria A, et al. (2020). Nutritional Impact on Embryo Implantation. Review of the Literature. J Nutrition Health Food Sci 8(2): 1-12. DOI: 10.15226/ jnhfs.2020.001178
19. Catharyn Stern, Larry Chamley. Antiphospholipid Antibodies and Coagulation Defects in Women with Implantation Failure After IVF and Recurrent Miscarriage. Reprod Biomed Online. 2006; 13(1): 29-37. doi:10.1016/s1472-6483(10)62013-5
20. DC Dutta's Textbook of Obstetrics; Dutta, D.C. and Konar; 2015; Jaypee Brothers Medical Publisher. Pg.29, 25
21. DC Dutta's Textbook of Obstetrics; Dutta, D.C. and Konar; 2015; Jaypee Brothers Medical Publisher. Pg. 39
22. Bernard Aurousseau, Dominique Gruffat, Denys Durand. Gestation Linked Radical Oxygen Species Fluxes and Vitamins and Trace Mineral Deficiencies in the Ruminant. Reprod Nutr Dev. 2006; 46(6): 601-620. doi: 10.1051/rnd:2006045

Dr. Arun M¹, Dr. Savitha HP²

¹Phd Scholar, Department of Kayachikitsa SDMT’s AMC Terdal

²Professor, Department of Manasaroga, SDM College of Ayurveda and Hospital – Hassan

http://dx.doi.org/10.47191/ijahm/v14i6.06

ABSTRACT: Madya is consider as alcohol in Ayurveda. Madya is mentioned in Brihattrayi and Laghuttrayi. According to Ayurveda if Madya is taken in proper manner and dose act as nectar (Amrit). If it is taken in excess doses it produces Mada (acute alcoholism) Madatyaya or Panatyaya (chronic alcoholism) and Panavibhrama (Alcohol withdrawal syndrome). There is no single cause of Alcoholism. Alcohol withdrawal is a set of symptoms seen after the cessation of heavy usage of Alcohol lasts to 24 hours to 72 hours and need a proper care. The symptoms varies person to person like insomnia, nausea, vomiting, headache, hallucinations, tremors, seizures and even leads to death. Various treatment modalities like Shodana and Shaman explained in Ayurveda to treat Madatyaya. They need proper nutritional supplementation in proper manner and should provide immediate nourishment to control the condition. Here the combination of 7 drugs which are rich in vitamins nutrients and minerals explained in authenticate text book of Ayurveda made in to syrup form for easy administration and storage and which effective in Madatyaya.

KEYWORD : Madatyaya, Alcohol Withdrawak syndrome, Kharjuradi Mantha Syrup.

REFERENCES:

1. Adapted with permission from American Psychiatric Association. Diagnostic and statistical manual of mental disorders. 4th ed., text revision. Washington, D.C.: American Psychiatric Association, 2000:216.
2. Sullivan JT, Sykora K, Schneiderman J, Naranjo CA, Sellers EM. Assessment of alcohol withdrawal: the revised clinical institute withdrawal assessment for alcohol scale (CIWA‐Ar). British journal of addiction. 1989 Nov;84(11):1353-7.
3. Bayard M, Mcintyre J, Hill KR, Jack Woodside JR. Alcohol withdrawal syndrome. American family physician. 2004 Mar 15;69(6):1443-50.
4. K.Sharma, Bhagawan Dash Vol.4 Charaka Samhita, Choukhamba Publication, 2015, Chikitsa stana 24 Chapter shloka107-108, page 410.
5. K.Sharma, Bhagawan Dash Vol.4 Charaka Samhita, Choukhamba Publication, 2015, Chikitsa stana 24 Chapter, Shloka195-196, page 428.
6. K.Sharma, Bhagawan Dash Vol.4 Charaka Samhita, Choukhamba Publication, 2015, Chikitsa stana 24 Chapter, shloka117-120, page 412.
7. K.Sharma, Bhagawan Dash Vol.4 Charaka Samhita, Choukhamba Publication, 2015, Chikitsa stana 24 Chapter, Shloka 195-196, page428.
8. K.Sharma, Bhagawan Dash Vol.4 Charaka Samhita, Choukhamba Publication, 2015, Chikitsa stana 24 Chapter, Shloka191-193, page 385-387.
9. Shambunath Pandey Shastri Virachita, Sushruta Samhita, Uttarardha, Krishna Das Academy Varanashi, 1985, 47 Chapter, Shloka, 24-25, page 684.
10. Shambunath Pandey Shastri Virachita, Sushruta Samhita, Uttarardha, Krishna Das Academy Varanashi, 1985, 47 Chapter, Shloka, 26-27, page 684.
11. Shambunath Pandey Shastri Virachita, Sushruta Samhita, Uttarardha, Krishna Das Academy Varanashi, 1985, 47 Chapter, Shloka, 27-28, page 684.
12. Shambunath Pandey Shastri Virachita, Sushruta Samhita, Uttarardha, Krishna Das Academy Varanashi, 1985, 47 Chapter, Shloka, 29, page 684.
13. Shambunath Pandey Shastri Virachita, Sushruta Samhita, Uttarardha, Krishna Das Academy Varanashi, 1985, 47 Chapters, Shloka, 34-36, page 685
14. Saitz R. Introduction to alcohol withdrawal. Alcohol health and research world. 1998;22(1):5.
15. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 409-10.
16. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 407-08.
17. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 422-23.
18. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 420-21.
19. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 404-05.
20. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 402-03.
21. Vinayak A. Bhavaprakasha nighantu of Bhavamisra. 1^st Chaukhambha orientalia; 2021. P 08-09.
22. Mishra et al. A Critical Review on KharjuradiManth - An AyurvedicPolyherbal Formulation for Alcohlism (Acute and Chronic Alcohol Addiction and Withdrawal) , 2020 Greentree Group Publishers © IJAPC Int J Ayu Pharm Chem 2018 Vol. 12 Issue 2
23. ShobhaG.Hiremath; A Text Book Of BhaishajyaKalpana(Indian Pharmaceutics);SharkaraKalpana:I B H Prakashana.2000;page no 129-30.
24. Javeed A, Chate V. A Clinical study to evaluate the role of Kharjooradi Mantha in the enhancement of Ojo Guna in Madatyayi subjects. Journal of Ayurveda and Integrated Medical Sciences. 2018 Oct 31;3(05):14-20.
25. Javeed A, Chate V. A Clinical study to evaluate the role of Kharjooradi Mantha in the enhancement of Ojo Guna in Madatyayi subjects. Journal of Ayurveda and Integrated Medical Sciences. 2018 Oct 31;3(05):14-20.

 Huziafa Ashfaq Salati¹, Mohd. Afsahul Kalam^*2, Tooba Bilal Wani³, Urzeeba Zahoor¹, Uzma Jan¹, Ajaz Ahmad Bhat¹

¹PG Scholar Department of Ilmul Advia, Regional Research Institute of Unani Medicine, University of Kashmir, Habak, Naseembagh Campus, Hazratbal, 190006, J&K, India.

²Research Officer Unani, Regional Research Institute of Unani Medicine, University of Kashmir, Habak, Naseembagh Campus, Hazratbal, 190006, J&K, India.

³PG Scholar Department of Moalajat, Regional Research Institute of Unani Medicine, University of Kashmir, Habak, Naseembagh Campus, Hazratbal, 190006, J&K, India.

^*Corresponding Author: Mohd. Afsahul Kalam

Research Officer Unani, Department of Ilmul Advia, RRIUM, University of Kashmir, Habak, Naseembagh Campus, Hazratbal, Srinagar, 190006, J&K;

http://dx.doi.org/10.47191/ijahm/v14i6.07

ABSTRACT: Kundur, the Indian olibanum tree, is a member of the Burseraceae family and grows best in the dry mountainous regions of the Middle East, Northern Africa, and India. This tree has small to huge branches. The tree's oleo-gum resin is removed and stored from the incision created in its trunk. Resin makes roughly 30–60% of oleo-gum resin, with organic solvent-soluble essential oils making up the remaining 5–10%. Gum-resin extracts from Boswellia serrata have long been used in traditional medicine to treat a variety of chronic inflammatory diseases. Four primary pentacyclic triterpenic acids found in the resinous component of Boswellia serrata are β-boswellic acid, acetyl-β-boswellic acid, 11-keto-β-boswellic acid, and acetyl-11-keto-β-boswellic acid. This oleo-gum resin is quite popular among traditional practitioners of traditional Chinese and Indian Systems of medicine because of its wide range of useful biological properties such as anti-inflammatory, anti-arthritic, anti-rheumatic, anti-diarrheal, anti-hyperlipidemic, anti-asthmatic, anti-cancer, anti-microbial, anti-fungal, anti-complementary, and analgesic activity etc.

KEYWORD : Kundur, Boswellia serrata, Unani, oleo-gum resin, Anti-arthritic

REFERENCES:

1. Lone AH, Ahmad T, Anwar M, Sofi G, Imam H and Habib S. Perception of health promotion in Unani herbal medicine. Journal of Ethnopharmacology Volume 276; 2021, 114144.

https://doi.org/10.1016/j.jep.2021.114144.

2. Makbul SAA, Jahan N and Ahmad G. Hajrul Yahood (Lapis judaicus): An important mineral drug of Unani system of medicine for the management of urolithiasis. Journal of Ethnopharmacology Vol 222; 2018, 165-170. https://doi.org/10.1016/j.jep.2018.04.047.
3. Kirtikar and Basu. Indian Medicinal Plants. Vol 1, 2^nd revised edn, 2012; 521-523.
4. Al-Harrasi A, Khan AL, Asaf S and Al-Rawahi A. Taxonomy, Distribution and Ecology of Boswellia. In: Biology of Genus Boswellia. Springer, Cham. 2019. https://doi.org/10.1007/978-3-030-16725-7_2.
5. Singh MP and Panda H. Medicinal herbs with their formulations. Daya Publishing House. Vol 1, 2005; 179-180.
6. The wealth of India. A Dictionary of Indian Raw Materials and Industrial Products. Revised edn. Publications & Information Directorate, CSIR, New Delhi. 1988, Vol 2. B: 203-209.
7. Rastogi RP and Mehrotra BN. Compendium of Indian Medicinal Plants- Vol 4. New Delhi: Central Drug Institute Lucknow and National Institute of Science Communication and Information Resources, 2008. 1985-1989: 114.
8. Rastogi RP and Mehrotra BN. Compendium of Indian Medicinal Plants-Vol 1. New Delhi: Central Drug Institute Lucknow and National Institute of Science Communication and Information Resources, 2008, 1960-1969: 61.
9. Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet; https://powo.science.kew.org/.
10. Hooker JD. The flora of British India. Part I. Ranunculaceae to Polygaleae. 528.
11. Mahe Alam, Hakimuddin Khan, Samiullah L and Siddique KM. A review on Phytochemical and Pharmacological studies of Kundur (Boswellia serrata Roxb ex Colebr.) -A Unani drug. Journal of Applied Pharmaceutical Science 2012; 02(03):148-156.
12. Handa SS and Kaul MK. Supplement to cultivation and utilization of medicinal plant. Regional Research Laboratory Council of Scientific and Industrial Research Jammu-Tawi 1996, 525-536.
13. Ghanī HN. Khazāin-ul-Advia. New Delhi: Idara Kitab-us-Shifa, 2011: 1069-1070.
14. Abdul Hakim HM. Bustān-ul-Mufaradāt Jadīd. Idara Kitab-us-shifa. New Delhi; 2015: 455.
15. Baytar I. Al-Jamia Li-Mufradāt al Advia wal Aghdhiya. Urdu translation. Central Council for Research in Unani Medicine. Vol. 4, 2003; 201-206.
16. Khare CP. Indian Medicinal Plants. An Illustrated Dictionary. Springer. 2007; 98-99.
17. Safi-uddin Ali HS. Unani Advia Mufrada. New Delhi: Qaumi Council baraye farog urdu zuban. 232-233.
18. Baghdādī HB. Kitāb-ul mukhtarāt fil tib. Urdu translated by CCRUM. Part II: New Delhi: Central Council for Research in Unani Medicine. 2005: 168.
19. Kabiruddin HM. Al-Qarābādīn. Central Council for Research in Unani Medicine. 2006; 83, 451, 543, 587, 1068, 1134, 1174, 1232.
20. Goswami HRL. Bayānul Advia. Manzoor shuda central council of Ayurvedic wa tib Unani and homeopathic. Idara Kitab us Shifa, New Delhi. 2019: 416.
21. The wealth of India. A Dictionary of Indian Raw Materials & Industrial Products. First supplement series. National Institute of Science Communication and Information Resources (NISCAIR). 2007, Vol. 1: A – Ci: 147.
22. Ahmed HH, Abd-Rabou AA, Hassan AZ and Kotob SE. Phytochemical Analysis and Anti-cancer investigation of Boswellia serrata bioactive constituents In Vitro. Asian Pac J Cancer Prev. 2015; 16 (16): 7179-7188. DOI: http://dx.doi.org/10.7314/APJCP.2015.16.16.7179. PMID: 26514509.
23. Singh GB and Atal CK. Pharmacology of an extract of salai guggul ex. Boswellia serrata. Indian J Pharmacol 1984; 16 :51.
24. Al-Harrasi A, Al-Rawahi A, Hussain J, Ali L, Hussain H, Rehman N et al. First in vitro lipid peroxidation and in vivo anti-inflammatory activity of the Omani frankincense of Boswellia sacra flueck. Int J Phytomed 2013; 5: 73-7.
25. Mothana RA. Anti-inflammatory, antinociceptive and antioxidant activities of the endemic Soqotraen Boswellia elongata balf. F. and Jatropha unicostata balf. F. In different experimental models. Food Chem Toxicol 2011; 49: 2594-9.
26. Sharma A, Bhatia S, Kharya MD, Gajbhiye V, Ganesh N, Namdeo AG, et al. Anti-inflammatory and analgesic activity of different fractions of Boswellia serrate. Int J Phytomed 2010; 2: 94-9.
27. Menon MK and Kar A. Analgesic and psychopharmacological effects of the gum resin of Boswellia serrata. Planta Med 1971; 19: 333-41.
28. Vongtau HO, Abbah J, Ngazal IE, Kunle OF, Chindo BA, Otsapa PB, et al. Anti-nociceptive and anti-inflammatory activities of the methanolic extract of Parinari polyandra stem bark in rats and mice. J Ethnopharmacol 2004; 90: 115-21.
29. Bishnoi M, Patil CS, Kumar A and Kulkani SK. Analgesic activity of acetyl-11-keto-b-boswellic acid, a lipooxygenase – Enzyme inhibitor. Indian J Pharmacol 2005; 37: 255-6.
30. Al-Harrasi A, Ali L, Hussain J, Rehman NU, Ahmed M and Al-Rawahi A. Analgesic effects of crude extracts and fractions of Omani frankincense obtained from traditional medicinal plant Boswellia sacra on animal models. Asian Pac J Trop Med 2014; 7: S485-90.
31. Chaurasia A and Gharia A. Antifungal activity of medicinal plant Boswellia serrata. J Ultra Chem 2017; 13: 88-90.
32. Garg SC. Antifungal activity of some essential oils. Indian J Pharmacol 1974; 36: 47.
33. Zutsi U, Rao PG and Kaur S. Mechanism of cholesterol lowering effect of Salai guggal ex-Boswellia serrata Indian J Pharmacol 1986; 18: 182-3.
34. Gerlach U. Sorbitol dehydrogenase. In: Bergmeyer HU, editor. Methods of Enzymatic Analysis. 3rd ed. Weinheim: Verlag Chemie; 1983. p. 112-7.
35. Azadmehr A, Ziaee A, Ghanei L, Fallah Huseini H, Hajiaghaee R, Tavakoli-Far B, et al. A randomized clinical trial study: Anti-oxidant, anti-hyperglycemic and anti-hyperlipidemic effects of Olibanum gum in type 2 diabetic patients. Iran J Pharm Res 2014; 13: 1003-9.
36. Shehata AM, Quintanilla-Fend L, Bettio S, Singh CB and Ammon HP. Prevention of multiple low-dose streptozotocin (MLD-STZ) diabetes in mice by an extract from gum resin of Boswellia serrata (BE). Phytomedicine 2011; 18:1037-44.
37. Zutshi U, Siddiqui M, Singh GB and Atal CK. Mechanism of Action of Salai Guggul as Antilipidmic, Agent Souvenier, 13th Indian Pharmacological Society, Conference; 1980. p. 1-31.
38. Zutshi U, Rao PG, Samagat K, Singh GB and Atal CK. Mechanism of cholesterol lowering effect of Salai guggal ex- Boswellia serrata. Indian J Pharm 1980; 12:59.
39. Liu JJ, Toy WC, Liu S, Cheng A, Lim BK, Subramaniam T, et al. Acetyl-keto-β-boswellic acid induces lipolysis in mature adipocytes. Biochem Biophys Res Commun 2013; 431:192-6.
40. Borrelli F, Capasso F, Capasso R, Ascione V, Aviello G, Longo R, et al. Effect of Boswellia serrata on intestinal motility in rodents: Inhibition of diarrhoea without constipation. Br J Pharmacol 2006; 148: 553-60.
41. Shah BA, Qazi GN and Taneja SC. Boswellic acids: A group of medicinally important compounds. Nat Prod Rep 2009; 26: 72-89.
42. Takada Y, Ichikawa H, Badmaev V and Aggarwal BB. Acetyl-11-ketobeta-boswellic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing NF-kappa B and NF-kappa B-regulated gene expression. J Immunol 2006; 176: 3127-40.
43. Liu JJ, Huang B and Hooi SC. Acetyl-keto-beta-boswellic acid inhibits cellular proliferation through a p21-dependent pathway in colon cancer cells. Br J Pharmacol 2006; 148: 1099-107.
44. Lu M, Xia L, Hua H and Jing Y. Acetyl-keto-beta-boswellic acid induces apoptosis through a death receptor 5-mediated pathway in prostate cancer cells. Cancer Res 2008; 68:1180-6.
45. Pang X, Yi Z, Zhang X, Sung B, Qu W and Lian X. Acetyl-11-keto-betaboswellic acid inhibits prostate tumor growth by suppressing vascular endothelial growth factor receptor 2-mediated angiogenesis. Cancer Res 2009; 69: 5893-900.
46. Hostanska K, Daum G and Saller R. Cytostatic and apoptosis-inducing activity of boswellic acids toward malignant cell lines in vitro. Anticancer Res 2002; 22: 2853-62.
47. Liu JJ, Nilsson A, Oredsson S, Badmaev V and Duan RD. Keto- and acetyl-keto-boswellic acids inhibit proliferation and induce apoptosis in Hep G2 cells via a caspase-8 dependent pathway. Int J Mol Med 2002; 10: 501-5.
48. Glaser T, Winter S, Groscurth P, Safayhi H, Sailer ER and Ammon HP. Boswellic acids and malignant glioma: Induction of apoptosis but no modulation of drug sensitivity. Br J Cancer 1999; 80: 756-65.
49. Sinha VR, Singh A, Singh S and Bhinge JR. Compression coated systems for colonic delivery of 5-fluorouracil. J Pharm Pharmacol 2007; 59: 359-65.
50. Ahmed HH, Rahman MA, Zahraa F, Shalby A and Lokman MS. Antitumor efficacy of Boswellia serrata extract in management of colon cancer induced in experimental animal. Int J Pharm Sci 2013; 3: 379-89.
51. Ismail1 SM, Aluru S, Sambasivarao KR and Matcha B. Antimicrobial activity of frankincense of Boswellia serrata. Int J Curr Microbiol Appl Sci 2014; 3: 1095-101.
52. Patel NB and Patel KC. Antibacterial activity of Boswellia serrata Ex colebr. ethanomedicinal plant against gram negative UTI pathogens. Life sciences leaflets. 2014; 53:976-1098.
53. Baratta MT, Dorman HJ, Deans SG, Figueiredo AC, Barroso JG and Ruberto G. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragrance J 1998; 13: 235-40.
54. Raja AF, Ali F, Khan IA, Shawl AS, Arora DS, Shah BA, et al. Antistaphylococcal and biofilm inhibitory activities of acetyl-11-ketoβ-boswellic acid from Boswellia serrata. BMC Microbiol 2011; 11: 54.