^Back To Top
Volume & Issue : Volume 4 Issue 5
Therasilin Louis*, Sonya Cheriyan, Nishanth Gopinath
Experimental Pharmacology Unit, Nagarjuna Herbal Concentrates Ltd, Kalayanthani, Thodupuzha,
Idukki-685 588, INDIA
* Corresponding Author
Dr. Therasilin Louis
Experimental Pharmacology Unit
Nagarjuna Herbal Concentrates Ltd
Idukki – 685 588
Amruth Jeevan Rasayan is comprising of the purely herbal ‘avaleha’ / herbal jam named Amruth Jeevan Rasayan Avaleha along with the mineral-rich Amruth Jeevan Rasayan Tablets, formulated following the principles of treating aging and age-associated diseases. Being an anti aging drug, Amruth Jeevan Rasayan must have antioxidants and free radical scavenging activity to minimize free radical-induced damage, which is a key cause of aging. The methanolic extract of Amruth Jeevan Rasayan was evaluated in vitro for total phenolic and free radical scavenging activity. The total phenolic content was measured using Folin-ciocalteu reagent against gallic acid. Free radical scavenging activity was measured by 2,2-diphenyl-1-picryl hydroxyl (DPPH) and 2,2’-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) di ammonium salt (ABTS) assay. The studies showed that Amruth Jeevan Rasayan possesses antioxidant activity. The results of this study suggest that the antioxidant and free radical scavenging activity of Amruth Jeevan Rasayan may explain its Rasayan effect and justify its use as a medicine for age associated diseases.
Antioxidant, Phytochemicals, Amruth Jeevan Rasayan, DPPH, and ABTS
1. 1. Sreedhar V, Ravindra Nath LK, Madana Gopal N, Sanjith Nath M. In-vitro antioxidant activity and free radical scavenging potential of roots of Vitex trifoliate Res J Pharm, Biol and Che Sci 2010; 1(4):1036-1044.
2. Halliwell B, Gutteridge JMC. Oxygen toxicity, oxygen radicals, transition metals and disease. J Biochem 1984; 219:1–14.
3. Simonian NA, Coyle JT. Oxidative stress in neurodegenerative diseases. Annu Rev Pharmacol Toxicol 1996; 36: 83–106.
4. Dhalla NS, Temsah RM, Netticadan T. Role of oxidative stress in cardiovascular diseases. J Hypertens 2000;18:655–673.
5. Vant Veer P, Jansen MC, Klerk M, Kok FJ. Fruits and vegetables in the prevention of cancer and cardiovascular disease. Public Health Nutr 2000;3:103–107.
6. Bokov A, Chaudhuri A, Richardson A. The role of oxidative damage and stress in aging. Mech Ageing Dev 2004;125:811–826.
7. Madamanchi NR, Vendrov A, Runge MS. Oxidative stress and vascular disease. Arterioscler. Thromb Vasc Biol 2005; 25:29–38.
8. Fritz KL, Seppanen CM, Kurzer MS, Csallany AS: The in vivo antioxidant activity of soybean isoflavone in human subjects. Nut Res 2003;23:479–487.
9. Patricia I, Oteiza AG, Erlejman S, Verstraeten V, Keen CL, Fraga CS. Flavonoid membrane interactions: A protective role of flavonoids at the membrane surface. Clin Develop Immunol 2005;12:23–25.
10. Bravo L. Polyphenol: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 1998;56:317–333.
11. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J Nutr Biochem 2002;13:572–584.
12. Pier-Giorgio P. Flavonoids as antioxidants. J Nat Prod 2000;63:1035–1042.
13. Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenoilc compounds. Trends Plant Sci 1997;2:152–159.
14. Sealbert A, Johnson J, Saltmarsh M. Polyphenols: Antioxidants and beyond. Am. J Clin Nutr 2005;81:2155–2175.
15. Ross JA, Kasum CM. Dietary flavonoids: Bioavailability, metabolic effects, and safety. Ann Rev Nutr 2002;22:19–34.
16. Rice-Evans CA, Miller N. Maxwell SJ. Prospects for the use of antioxidant therapies. Drugs1995;49:345.
17. Ames SN, Shigrenaga MK, Hagen TM. Oxidant, antioxidant and degradative disease of aging. Proc Nat Acad Sci USA 1993;90:7915–7922.
18. Robak J, Gryglewski RJ. Flavonoids are scavengers of superoxides anions. Biochem Pharmacol 1988;37:837–841.
19. Ardestani A, Yazdanparast R. Antioxidant and free radical scavenging potential of Achillea santolina extracts. Food Chem 2007;104:21–29.
20. Bingham M, Gibson G, Gottstein N, Pascual-Teresa SD, Minihane AM, Rimbach G: Gut metabolism and cardio protective effects of dietary isoflavones. Current Top Nut Res 2003;1:31–48.
21. Silva BM, Andrade PB, Valentãoo P, Ferreres F, Seabra RM, Ferreira MA: Quince (Cydonia oblonga Miller) fruit (pulp, peel, and seed) and jam: antioxidant activity. J Agric Food Chem 2004;52:4705–4712.
22. Shasthri K, Chaturvedi G. Charaka Samhita with Vidyotini Commentoty. Varanasi: Chaukambha Bharati Academy 2007;pp.35–6.
23. Govindarajan R, Vijayakumar M, Pushpangadan P. Antioxidant approach to disease management and the role of ‘Rasayana’ herbs of Ayurveda. Journal of Ethnopharmacology 2005;99:165–178.
24. Baxi AJ, Shukla VJ, Bhatt UB. Guideli Monograph on Testing of Some Ayurvedic Formulations. Jamnagar: Gujarat Ayuurveda University; 2002.p.5-15.