Arpita Roy and Navneeta Bharadvaja*

DOI : http://dx.doi.org/10.18535/ijahm/v7i4.02

Plant Biotechnology Laboratory, Department of Biotechnology, 

Delhi Technological University, New Delhi-110042, India

ABSTRACT:

The objective of the study was to assess the effect of three plant tissue culture media (MS, Gamborg’s B5 and Nitsch) on the growth of five accessions of Centella asiatica using nodal explant. All the three media supplemented with 1 mg/l BAP resulted in increased growth. Amongst the three Medias, MS media resulted in the highest shoot multiplication in all the five accessions after eight weeks of the incubation period.  The highest number of shoots and maximum shoot length were obtained in accession number 342109 (19.6±0.57 shoot number, 4.9±0.53 shoot length) grown in MS media supplemented with 1 mg/l BAP. Estimation of stigmasterol, an important phytosterol present in the leaves (main part for synthesis) was done by using gas chromatography and the highest amount of stigmasterol was obtained in accession number 342109 i.e. 0.0228µg/mg of leaves.

Keywords- Centella asiatica; MS media; B5 media; Nitsch media; Stigmasterol; Gas Chromatography

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