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Keshwar Unmesh*, Pimplapure Soumit, Dhurde Ss, Shrikhande Bk
Siddhayu Ayurvedic Research Foundation Pvt. Ltd, Nagpur, India.
Aim: The present study was designed with an objective of developing a validated, simple, accurate and precise HPTLC method for the determination of Diosgenin from Polyherbal Tablet containing Tribulus terrestris.
Method: High performance thin layer chromatography (HPTLC) method was developed and validated for analysis of Diosgenin from polyherbal Tablet. Chromatographic separation was achieved on precoated silica gel HPTLC aluminum plate 60 F254 using Toluene: Acetone (8.5:1.5) as mobile phase. Detection was performed at 430 nm wavelength by TLC scanner (CAMAG).
Results: The Rf value of Diosgenin was found to be 0.41 ± 0.02. The linear regression analysis data for calibration plots showed good linear relationship (r2 = 0.997) in the concentration range of 240 - 1440 ng/spot. The accuracy of the method was checked by conducting recovery studies at three different levels, using standard addition method. The average recovery of diosgenin was found to be 98.48 %. Precision (% RSD < 2%) of the developed HPTLC method was in accordance with the ICH guidelines.
Conclusion: The developed procedure can be selectively used in the assay and quality control of Diosgenin from various Ayurvedic Polyherbal Tablet formulations without interference from other herbal ingredients and excipients.
1. Indian Herbal Pharmacopoeia. Revised new Edn, Indian Drug Manufacturers Association, Mumbai, 2002, 459-466.
2. Rajpal V. Standardisation of Botnicals. Vol. I, Eastern publishers, New Delhi, 2002,
3. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Edn 33, Nirali Prakashan, Pune, 2005, 225-226.
4. The Useful Plants of India. Edn 3, Publications & Information Directorate, CSIR, New Delhi, 1994, 647-648.
5. Nadkarni KM. Indian Materia Medica. Edn 3, Vol. I, Popular Prakashan, Mumbai, 1976, 1229-1230.
6. Handa SS, Kapoor VK. Pharmacognosy. Edn 2, Vallabh Prakashan, Delhi, 1988, 229-230.
7. The Ayurvedic Pharmacopoeia of India. Edn 1, Part I, Vol. I, The Controller of Publications, Delhi, 1990, 40.
8. Dey AC. Indian Medicinal Plants used in Ayurvedic Preparations. Bishen Singh Mahendra Pal Singh, Dehra Dun, 1994, 52-53.
9. Tirtha SS. The Ayurveda Encyclopedia Natural Secrets to Healing, Prevention, & Longevity. Edn 1, Sri Satguru Publications, Delhi, 1998, 86.
10. Kshirsagar VB, Deokate UA, Bharkad VB, Khadabadi SS. HPTLC Method Development and Validation for the simultaneous Estimation of Diosgenin and Levodopa in marketed formulation. Asian Journal Research Chemistry 2008; 1(1): 36-39.
11. Parameswaran S, Koshti S. Quantitative Estimation of Piperine and Diosgenin in Ayurvedic formulation. British Journal of Pharmaceutical Research 2014; 4(8): 911-920.
12. Warke VB, Deshmukh TA, Patil VR. Development and Validation of RP-HPLC Method for Estimation of Diosgenin in Pharmaceutical dosage form. Asian Journal of Pharmaceutical & Clinical Research 2011; 4 (1): 126-128.
13. Ghodasara T, Gaudani R, Nayak B, Shingala D. HPTLC Method Development and Validation for the Simultaneous Estimation of Diosgenin and Gallic acid in marketed formulation. Pharma Science Monitor An International Journal of Pharmaceutical Science 2012; 3(4): 2664.
14. Jayaprakasm R, Ravit K, Athira K, Asha JB. Quantitative Determination Of Diosgenin In Polyherbal Formulation & Various Extracts of Smilax china Linn using standard marker by Validated Analytical Techniques. Universal Journal of Pharmacy 2013; 02(04): 83-90.
15. ICH Harmonized Tripartite Guideline. Validation of Analytical Procedures: Text and Methodology. Q2 (R1), November 2005.