Keshwar Unmesh^*, Pimplapure Soumit, Dhurde Ss, Shrikhande Bk

Paper Index : 03.2015-52324687

Siddhayu Ayurvedic Research Foundation Pvt. Ltd, Nagpur, India.

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Abstract

      Aim: The present study was designed with an objective of developing a validated, simple, accurate and precise HPTLC method for the determination of Diosgenin from Polyherbal Tablet containing Tribulus terrestris.

Method: High performance thin layer chromatography (HPTLC) method was developed and validated for analysis of Diosgenin from polyherbal Tablet. Chromatographic separation was achieved on precoated silica gel HPTLC aluminum plate 60 F₂₅₄ using Toluene: Acetone (8.5:1.5) as mobile phase. Detection was performed at 430 nm wavelength by TLC scanner (CAMAG).

Results: The R_f value of Diosgenin was found to be 0.41 ± 0.02. The linear regression analysis data for calibration plots showed good linear relationship (r² = 0.997) in the concentration range of 240 - 1440 ng/spot. The accuracy of the method was checked by conducting recovery studies at three different levels, using standard addition method. The average recovery of diosgenin was found to be 98.48 %. Precision (% RSD < 2%) of the developed HPTLC method was in accordance with the ICH guidelines.

Conclusion: The developed procedure can be selectively used in the assay and quality control of Diosgenin from various Ayurvedic Polyherbal Tablet formulations without interference from other herbal ingredients and excipients.

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