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Impact Factor : 4.415 ...... IJAHM Latest Issue IJAHM have published its latest issue Vol. 8 Issue 02 2018 published IJAHM Call For Paper invites paper from various Ayurveda & herbal Medicine for Volume 8 Issue 02 March-April. 2018

Keshwar Unmesh*, Pimplapure Soumit, Dhurde Ss, Shrikhande Bk

Paper Index : 03.2015-52324687

Siddhayu Ayurvedic Research Foundation Pvt. Ltd, Nagpur, India.



      Aim: The present study was designed with an objective of developing a validated, simple, accurate and precise HPTLC method for the determination of Diosgenin from Polyherbal Tablet containing Tribulus terrestris.

Method: High performance thin layer chromatography (HPTLC) method was developed and validated for analysis of Diosgenin from polyherbal Tablet. Chromatographic separation was achieved on precoated silica gel HPTLC aluminum plate 60 F254 using Toluene: Acetone (8.5:1.5) as mobile phase. Detection was performed at 430 nm wavelength by TLC scanner (CAMAG).

Results: The Rf value of Diosgenin was found to be 0.41 ± 0.02. The linear regression analysis data for calibration plots showed good linear relationship (r2 = 0.997) in the concentration range of 240 - 1440 ng/spot. The accuracy of the method was checked by conducting recovery studies at three different levels, using standard addition method. The average recovery of diosgenin was found to be 98.48 %. Precision (% RSD < 2%) of the developed HPTLC method was in accordance with the ICH guidelines.

Conclusion: The developed procedure can be selectively used in the assay and quality control of Diosgenin from various Ayurvedic Polyherbal Tablet formulations without interference from other herbal ingredients and excipients.



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Crossref - DOI

DOI : 10.31142/ijahm

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