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IJAHM Latest Issue IJAHM have published its latest issue Vol. 7 Issue 04 2017 published IJAHM Call For Paper invites paper from various Ayurveda & herbal Medicine for Volume 7 Issue 04 July-Aug. 2017 ...... Impact Factor : 4.415

Dr. Ninad Sathe1, Dr. Rohit Chavan2, Dr. Mujahid Khan3, Dr. Ashish punde4

 Paper Index :

 1.Professor, Dept. of Rasashastra & Bhaishajyakalpana, YMT Ayurvedic Medical College, Kharghar, Navi Mumbai,

2,3.MD (Rasashastra & Bhaishajyakalpana)

4.Assistant professor, Dept. of Rasashastra & Bhaishajyakalpana, YMT Ayurvedic Medical

 College, Kharghar, Navi Mumbai,

Abstract

Herbo-mineral formulations are gaining negative propaganda in population. Hence, mankind can remain deficient from the bounty of effective and efficient ayurvedic formulation like makardhawaja. It is used as a rejuvenating & aphrodisiac (rasayan and vajikaran) drug. Modern medicine evolved a method of subjecting all assumptions to experimental verification and statistical validation. Therefore, current research had been undertaken with an aim to study cytotoxicity against selected MCF-7 human cell lines. Makardhwaj was prepared according to the reference of rasachandanshu which is a herbo-mineral formulation. Its cytotoxicity study was conducted at ACTREC. The solvent used for dissolution of the study drug was DMSO. Human cancer cell lines MCF-7 of breast were cryopreserved in liquid nitrogen and DMSO at -20 0C. Cell culture was performed in incubators at 37°C .The selected cell lines were inoculated in 96 well plates, in which study drug was tested at 4 dose levels i.e. 10, 20, 40, 80 µg/ml. Adriamycin was used as a positive control drug for comparative screening. According to SRB assay protocol Lc50values were calculated as declared by NCI. The study had revealed that, makardhwaja is not cytotoxic as the observed LC50 values were found > 80 in the study.

      References

1. Makardhwaja through Ages. Dr.Debasispanigrahi, Dr.Parimi Suresh.. Journal Of Drug Research 2013:2(1):36 42. ISSN: 2319-5916.

2. Rasachandanshuramesh Vitthalsuryawanshishreegajanan Book Depo, Sadashivpeth,    Pune, Third Print.

3. SRB ASSAY-Skehan P, Storeng R, Scudiero D, Monks A, Mcmohan J, Vistica D, Warrenjt, Bokesh H, Kenny S & Boyd M, New Calorimetric Cytotoxicity Assay For Anticancer Drugscreening. J. Natl. Cancer Inst, 82, 1990, 1107-1111.

4.Sulforhodamine B Colorimetric Assay For Cytotoxicity Screening Nature Protocols 1, - 1112 - 1116 (2006) Vanichavichai And Kanyawimkirtikara.

5.S Sinyorita, CK Ghosh, A Chakrabarti, B Auddy, Runaghosh, PK Debnath, - A Preliminary Study, IJEB, Vol. 49, July 2011, Pg- 534-539.

6. MATERIA MEDICA.

7. Sushrutsamhita,Nidansthan, Shastri AD, Part- I, 11th Edn, (Choukhamba Sanskrit Sansthan , Varanasi), 1997, 272-273.

8.Toxicity Tests With Mammalian Cell Culture

B. EKWALL, V. SILANO, A. PAGANUZZI-STAMMATI AND F. ZUCCO

Short-Term Toxicity Tests for Non-Genotoxic Effects Edited By P. Bourdeau Et Al. @1990 SCOPE. Published By John Wiley & Sons Ltd.

9.Rasataranginisadananda Sharma Kashinathshashtrimotilalbanarasi Das 2000.

10.Aayurved Prakash, Shree Gulabraj Sharma Mishra., 3rd Edition.

11.Yogratnakar II, Bhisagratnabrahmashankarshastrichoukhambaprakashan.

12.Evaluation Of In Vitro Anticancer Activity of Hydroalcoholic Extract Of Taebernaemontana Divaricate. Akhilasravyadantu, Shankarguru P, Ramya Devi D, Vedhahari BN* Department Of Pharmaceutical Technology, School Of Chemical and Bio-Technology, SASTRA University, Thanjavur, 613401, (T.N.), India.

13.Evaluation Of Some Plant Extracts For Standardization And Anticancer Activity SN Gaidhani1*, Arjun Singh1, Suman Kumari1, GS Lavekar1, AS Juvekar2, S Sen2 & MM Padhi1 1Central Council For Research In Ayurvedic Sciences, 61-65, Institutional Area, Opposite ‘D’ Block, Janakpuri, New Delhi;  2Advance Centre For Treatment, Research And Education In Cancer (ACTREC), Kharger, Navi Mumbai-410 210, India.

14.Anticancer Potential Of The Convolvulus Arvensismanbir Kaur1, A.N.Kalia2 I.S.F. College Of Pharmaceutical Sciences And Research, Moga1,2 Punjab.

15.Anticancer And Antioxidant Activities Of Standardized Whole Fruit, Pulp, And Peel Extracts Of Egyptian Pomegranate  Amira Abdel Motaal*,A And Sherifshakerb A Pharmacognosy Department, Faculty Of Pharmacy, Cairo University, Kasr-El-Ainy St., Cairo 11562, Egypt Bpharmaceutical Research And Development Department, Atos Pharma, Sekem, 3 Cairo-Belbeis Road, El Horreya, Cairo, P.B. 2834, Egypt.

16.Anti-Cancer And Anti-Microbial Activity Of Hydro Alcoholic Extract Of Bougainvillea Glabra Joshny J, Ramya Devi D, *Vedhahari B.N   Department Of Pharmaceutical Technology, School Of Chemical And Bio-Technology, SASTRA University, Thanjavur, 613401, (T.N.). India.

17.The NCI In Vitro Anticancer Drug Discovery Screen Concept, Implementation, And Operation, 1985-1995.Michael R. Boyd, MD, Phd

18.Methods For In Vitro Screening Of Anticancer Agents S. K. Kanthilal1, V.Suresh1, G. Arunachalam2, A. Suresh1, G. Shekar1, N. Senthilkumar1. 1 J.K.K. Munirajah Medical Research Foundation College Of Pharmacy, B.Komarapalayam, Nammakal.2

a.       PGP College of Pharmaceutical Sciences and Institute Nammakal, Tamilnadu

b.      JKKMMRF College of Pharmacy

19.In Vitro Methods For Nanotoxicity Assessment: Advantages And Applications:Poonamtakhar And Sheefalimahant , MM College Of Pharmacy, MM University, Mullana-Ambala, Haryana, India

 

 

 

 

 

 

 

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Crossref - DOI

DOI : 10.18535/ijahm

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