Zúñiga-Martínez M L¹, Terán-Figueroa Y², Vértiz-Hernández A A³, Alcántara-Quintana L E⁴

DOI : http://dx.doi.org/10.31142/ijahm/v9i4.07

¹Doctorado en Ciencias Biomédicas Básicas, Universidad Autónoma de San Luis Potosí

²Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí

³Coordinación Académica Región Altiplano, Universidad Autónoma de San Luis Potosí

⁴Cátedra CONACYT, adscrita a Facultad de Enfermería y Nutrición, Universidad Autónoma

de San Luis Potosí

*Corresponding author: Alcántara-Quintana L E

Cátedra CONACYT, adscrita a Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí  

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Abstract

Introduction: Some varieties of garlic - such as the Snow Mountain variety - have been attributed a greater amount of medicinal effects than the rest of the Allium genus, due mainly to the amount of sulfur compounds they contain. The amount of these compounds depends - among other circumstances - on the extraction conditions and techniques.

Objective: To assess the effects of Snow Mountain garlic extracts, obtained by different processes, on count and cell viability in Cervicouterine Cancer cell lines.

Material and methods: Three extraction methods were used to obtain sulfur compounds; three different concentrations of each extract were prepared and their effects were evaluated on two neoplastic and one non-neoplastic cell lines. The evaluation methods were Neubauer chamber cell count at 24, 48 and 72 hours and a MTT test at 72 hours.

Results: A significant dose-dependent increase in the number and viability of neoplastic cells was observed (p = 0.0001 and p = 0.0003 respectively), specially in soxhlet extraction treatments.

Conclusion: the ethanolic, chloroformic and aqueous Snow Mountain garlic extractions show an increase in the number and viability of Cervicouterine Cancer (CuCa) cells, contrary to other Allium varieties, so their probable anti-neoplastic activity requires further study.

Keywords: cancer, Allium sativum L., Snow Mountain garlic, cell count and viability.

References

1.   Artacho RM, Olea MF, Ruiz MD. Determination of organic sulphur compounds in garlic extracts by gas chromatography mass spectrometry. Food Chem1995; 53: 91-93.

2.    Baghalian K, Ali SZ, Reza MN, Naghdi HB, Khalighi A. Evaluation of allicin content and botanical traits in Iranian garlic (Allium sativum L.) ecotypes. SciHortic 2005; 103: 155-166.

3.      Block E. The organosulfur chemistry of the genus Allium – Implications for the organic chemistry of sulfur. AngewChemInt Ed 1992; 31: 1135–1178.

4.      Bocchini P, Andalo C, Ppzzi R, Glleti G, Antonelli A. Determination of diallylthiosulfinate (allicin) in garlic (Allium sativum L.) by high-performance liquid chromatography with a post-column phytochemical reactor. Anal ChimActa 2001; 441: 37-43.

5.     Borlinghaus J, Albrecht F, Gruhke MC, Nwachukwu ID, Slusarenko AJ. Allicin: chemistry and biological properties. Molecules 2014; 19(8): 12591-12618. doi: 10.3390/molecules190812591.

6.      Bose S, Laha B, Banerjee S. Quantification of allicin by high performance liquid chromatography-ultraviolet analysis with effect of post-ultrasonic sound and microwave radiation on fresh garlic cloves. Pharmacogn Mag 2014; 10(Suppl 2): S1288-S293. doi: 10.4103/0973-1296.133279.

7.      Cavallit, CJ, Bailey JH. Allicin, the antibacterial principle of Allium sativum. Isolation, physical properties and antibacterial action. J Am ChemSoc 1944; 66(11): 1944–1952.

8.      Chen X, Pang S, Lin J, Xia J, Wang Y. Allicin prevents oxidized low-density lipoprotein-induced endothelial cell injury by inhibiting apoptosis and oxidative stress pathway. BMC Complement Altern Med 2016; 16:133. doi 10.1186/s12906-016-1126-9.

9.      Chhouk K, UemoriCh, Wahyudiono, Kanda H, Goto M. Extraction of phenolic compounds and antioxidant activity from garlic husk using carbóndioide expanded ethanol. ChemEng Process 2017; 117: 113-119.

10.  Diaz LJ, Jiménez KL. Validación de unmétodo de extracción de alicina en ajo y sucuantificaciónpor HPLC. Simposio de Metrología, SM2008-S2A2-1066-1 2008. Recuperado de:

http://cenam.gob.mx/simposio2008/sm_2008/memorias/S2/SM2008-S2A2-1066.pdf.

11.  Gruhlke MC, Nicco C, Batteux F, Slusarenko AJ. The Effects of Allicin, a Reactive Sulfur Species from Garlic, on a Selection of Mammalian Cell Lines. Antioxidants 2017; 6(1). doi: 10.3390/antiox6010001.

12.  Hassan H, Haiping W, Xinya L, Xixiang L. Impact of genetic factor and geographical location on allicin content of garlic (Allium sativum) Germplasm from Egypt and China. Inter J of Agri and Bio 2015; 17(1): 156-162.

13.  Ilic D, et al. Transformation of synthetic allicin: the influence of ultrasouns, microwaves, different solvents and temperatures, and the products isolation. ScientificWorldJournal 2012; 2012: 561823. doi: 10.1100/2012/561823.

14.  Mujica H, Pérez M, Sanabria M, Giménez A. Efecto de la densidad de siembra, fertilizaciónpotásica y almacenamiento de los bulbossobre la concentración de alicina en ajocriollomorado (Allium sativum L.) determinadamediante HPLC. RevistaCientífica UDO Agrícola 2013; 13(1): 128-134.

15.  Kamenetsky R, et al. Diversity in fertility potential and organo-sulphur compounds among garlics from Central Asia. BiodiversConserv 2005; 14(2): 281‒295.

16.  Lawson LD, Wood SG, Hughes BG. HPLC analysis of allicin and other thiosulfinates in garlic clove homogenates. Planta Med 1991; 57(3):263-70.

17.  Lawson LD, Wang ZJ, Hughes BG. Identification and HPLC quantitation of the sulfides and dialk(en)ylthiosulfinates in commercial garlic products. Planta Med 1991; 57(4): 363-70.

18.  Li T, Shi HY, Hua YX, Gao C, Xia Q, Yang G, Li B. Effects of allicin on the proliferation and cell cycle of chondrocytes. Int J ClinExpPathol 2015; 8(10): 12525-12532.

19.  Lutomski J. Components and biological properties of some Allium species. Poznan: Institute of the Medicinal Plants 1987; 1-58.

20.  Mahajan R, Sharma K, Bandryal S, Jamwal P, Billowria P. In vitro propagation and cryopreservation of Snow Mountain garlic endemic to Himalayan region. Int J AdvBiotechnol Res 2013; 4(3): 372-379.

21.  Mehri F, Auger JA, Rendu F, Bauvois B. Allium compounds, dipropyl and dimethyl thiosulfinates as antiproliferative and differentiating agents of human acute myeloid leukemia cell lines. Biologics 2008; 2(4): 885-895.

22.  Méndez LL, Castaigne F. Effect of temperature cycling on allinase activity in garlic. Food Chem 2008; 111(1): 56-60.

23.  Miron T, Wilchek M, Sharp A, Nakagawa Y, Naoi M, Nozawa Y, Akao Y. Allicin inhibits cell growth and induces apoptosis through the mitochondrial pathway in HL60 and U937 cells. J NutrBiochem 2008; 19(8): 524-535.

24.  Ruiz-Cabello L, Puerto M, Gutiérrez-Praena D, Pichard S, Cameán A. Estudioin vitro de la viabilidad de células Caco-2 en presencia de componentes de aceite de Allium spp. Revista de Toxicología (en línea) 2013; 30(2): 144-148.Disponible en: http://www.redalyc.org/articulo.oa?id=91931189004

25.  Santhosha SG, Jamuna P, Prabhavathi SN. Bioactive components of garlic and their physiological role in health maintenance: a review. Food Biosc 2013; 3: 59-74.

26.  Singh VK, Singh DK. Pharmacological effects of garlic (Allium sativum L.). ARBS Annual Review of Biomedical Sciences 2008; 10: 6-26.

27.  Slusarenko AJ, Patel A, Portz D. Control of plant diseases by natural products: allicin from garlic as a case study. Eur. J. Plant Pathol 2008; 121:313–322. doi.org/10.1007/s10658-007-9232-7.

28.  Soto VV, González R, Sance M, Burba L, Camargo A. Efecto de la interaccióngenotipo-ambientesobre la expresión del contenido de allicina y ácidopirúvico en ajo (Allium sativum L.). Rev FCA UNCuyo 2010; 42(2): 15-22.

29.  Sundaram SG, Milner JA. Diallyl disulfide induces apoptosis of human colon tumor cells. Carcinogenesis 1996; 17(4): 669-673.

30.  Wang H, Li X, Liu S, Jin S. Quantitative determination of allicin in Allium sativum L. bulbs by UPLC. Chrom 2010; 71(1-2):159–161.

31.  Wang H, et al. Influence of pH, concentration and light on stability of allicin in garlic (Allium sativum L.) aqueous extract as measured by UPLC. J Sci Food Agric 2015; 95(9): 1838-1844.

32.  Yu TH, Wu CM, Ho CT. Volatile compounds of deep-oil fried, microwave heated and oven-baked garlic slices. J Agric Food Chem 1993; 41(5): 800–805.

33.  Zhang Q, Yang D. Allicin suppresses the migration and invasion in cervical cancer cells mainly by inhibiting NRF2. ExpTher Med 2019; 17(3): 1523-1528.

34.  Zahoor A. In vitro studies on mass propagation and secondary metabolite enhancement in two important medicinal plant: Allium sativum var. Snow Mountain garlic and Lavateracashmeriana (an endangered medicinal plant). Thesis doctoral in botany of Sam Higginbottom University of Agriculture, Technology and sciences 2018.